Document Detail

Dietary phytosterol does not accumulate in the arterial wall and prevents atherosclerosis of LDLr-KO mice.
MedLine Citation:
PMID:  24267264     Owner:  NLM     Status:  In-Data-Review    
SCOPE: There have been conflicting reports on the usefulness of phytosterols (PS) in preventing atherosclerosis. We evaluated the effects of dietary PS supplementation in LDLr-KO male mice on the plasma and aorta sterol concentrations and on atherosclerotic lesion development.
METHODS AND RESULTS: Mice were fed a high fat diet (40% of energy) supplemented with or without PS (2% w/w, n = 10). Plasma and arterial wall cholesterol and PS concentrations, lesion area, macrophage infiltration, and mRNA expression from LOX-1, CD36, ABCA1 and ABCG1 in peritoneal macrophages were measured. After 16 weeks, the plasma cholesterol concentration in PS mice was lower than that in the controls (p = 0.02) and in the arterial wall (p = 0.03). Plasma PS concentrations were higher in PS-fed animals than in controls (p < 0.0001); however, the arterial wall PS concentration did not differ between groups. The atherosclerotic lesion area in the PS group (n = 5) was smaller than that in controls (p = 0.0062) and the macrophage area (p = 0.0007). PS correlates negatively with arterial lipid content and macrophage (r = -0.76; p < 0.05). PS supplementation induced lower ABCG1 mRNA expression (p < 0.05).
CONCLUSIONS: Despite inducing an increase in PS plasma concentration, PS supplementation is not associated with its accumulation in the arterial wall and prevents atherosclerotic lesion development.
Renata P A Bombo; Milessa S Afonso; Roberta M Machado; Maria Silvia Ferrari Lavrador; Valéria S Nunes; Eder R Quintão; Marcia Koike; Sergio Catanozi; Chin Jia Lin; Edna R Nakandakare; Ana Maria Lottenberg
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Publication Detail:
Type:  Journal Article     Date:  2013-10-25
Journal Detail:
Title:  Atherosclerosis     Volume:  231     ISSN:  1879-1484     ISO Abbreviation:  Atherosclerosis     Publication Date:  2013 Dec 
Date Detail:
Created Date:  2013-11-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0242543     Medline TA:  Atherosclerosis     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  442-7     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.
Endocrinology and Metabolism Division, Faculty of Medical Sciences of the University of São Paulo, Lipids Laboratory, LIM10, São Paulo, SP, Brazil.
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