| Dietary phospholipid-dependent reductions in gene expression and activity of liver enzymes in fatty acid synthesis in fasted-refed rats. | |
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MedLine Citation:
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PMID: 10524348 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effects of dietary soybean phospholipid, its hydrogenation product and safflower phospholipid on gene expression and the activity of hepatic enzymes in fatty acid biosynthesis were examined in fasted-refed rats. Phospholipid composition of soybean phospholipid and its hydrogenation product were the same, but the hydrogenation product contained negligible amounts of unsaturated fatty acids. Among phospholipid classes, lysophosphatidylcholine and phosphatidylinositol proportions were slightly higher in safflower phospholipid than in soybean phospholipid or its hydrogenation product. Rats were fasted for 2 d and refed a fat-free diet or a diet containing 4% fatty acids either as soybean oil or various phospholipid preparations for 3 d. Compared to the fat-free diet, the soybean oil diet only slightly decreased specific, but not total hepatic fatty acid synthetase and malic enzyme activity, and it was totally ineffective in modulating glucose 6-phosphate dehydrogenase and pyruvate kinase activity under our experimental conditions. The diets containing phospholipids, however, markedly decreased the activity of these enzymes. The extent of reduction was somewhat attenuated with hydrogenated soybean phospholipid as compared with soybean and safflower phospholipids. Dot and Northern blot hybridization using specific cDNA probes showed that, compared to a fat-free diet, diets containing phospholipids profoundly decreased the hepatic mRNA levels of enzymes in fatty acid synthesis. Soybean oil, however, only marginally affected these parameters. Hepatic mRNA levels for enzymes correlated well with enzyme activity. Dietary phospholipids therefore appear to have decreased enzyme activity in fatty acid synthesis primarily by suppressing the mRNA levels of these enzymes. Compared to soybean oil, hydrogenated soybean phospholipid is still effective in decreasing the activity and mRNA level of enzymes in fatty acid synthesis. Therefore, it is difficult to ascribe the potent physiological activity of phospholipid in reducing fatty acid synthesis entirely to polyunsaturated fatty acid moiety. |
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Authors:
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I A Rouyer; Y Takahashi; T Ide |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of nutritional science and vitaminology Volume: 45 ISSN: 0301-4800 ISO Abbreviation: J. Nutr. Sci. Vitaminol. Publication Date: 1999 Jun |
Date Detail:
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Created Date: 1999-11-17 Completed Date: 1999-11-17 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0402640 Medline TA: J Nutr Sci Vitaminol (Tokyo) Country: JAPAN |
Other Details:
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Languages: eng Pagination: 287-302 Citation Subset: IM |
Affiliation:
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Laboratory of Nutrition Biochemistry, Ministry of Agriculture, Forestry and Fisheries, Tsukuba Science City, Ibaraki, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Dietary Fats, Unsaturated / pharmacology* Fasting* Fatty Acid Synthetase Complex / genetics, metabolism Fatty Acids / biosynthesis* Food Gene Expression / drug effects* Glucosephosphate Dehydrogenase / genetics, metabolism Liver / enzymology* Lysophosphatidylcholines / analysis, pharmacology Malate Dehydrogenase / genetics, metabolism Male Phosphatidylinositols / analysis, pharmacology Phospholipids / pharmacology* Pyruvate Kinase / genetics, metabolism RNA, Messenger / metabolism Rats Rats, Sprague-Dawley Safflower Oil / chemistry Soybean Oil / chemistry |
| Chemical | |
Reg. No./Substance:
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0/Dietary Fats, Unsaturated; 0/Fatty Acids; 0/Lysophosphatidylcholines; 0/Phosphatidylinositols; 0/Phospholipids; 0/RNA, Messenger; 8001-22-7/Soybean Oil; 8001-23-8/Safflower Oil; EC 1.1.1.37/Malate Dehydrogenase; EC 1.1.1.49/Glucosephosphate Dehydrogenase; EC 2.7.1.40/Pyruvate Kinase; EC 6.-/Fatty Acid Synthetase Complex |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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