Document Detail


Dietary nucleotides accelerate intestinal recovery after food deprivation in old rats.
MedLine Citation:
PMID:  7782893     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Previous studies in very young rats have shown that dietary nucleotides improve small intestine repair after injury or malnutrition. To investigate the potential effect of nucleotides in old rats, which have a diminished capability for intestinal repair, 17-mo-old rats were deprived of food for 5 d and then fed a nucleotide-free diet or a nucleotide-supplemented diet for 3 or 6 d. Intestinal jejunal and ileal mucosal weight, protein and DNA were evaluated as intestinal growth markers, and brush-border maltase, sucrase, lactase and aminopeptidase activities were evaluated as intestinal differentiation markers. The adenine nucleotide pool and the adenylate energy charge were also evaluated as indices of nucleotide availability. Food deprivation significantly decreased mucosal growth markers as well as differentiation markers in both jejunum and ileum. The ATP pool was also significantly depressed, but the adenylate energy charge was not significantly altered. To a certain extent, refeeding restored the losses, but in the rats that were fed the nucleotide-free diet, the restoration of the jejunum was significantly slower and the restoration of the ileum differentiation markers was incomplete compared with the rats fed the nucleotide-supplemented diet. The results suggest that dietary nucleotide intake in the elderly may accelerate the normal physiological intestinal response to refeeding after food deprivation.
Authors:
M A Ortega; M C Nunez; A Gil; A Sánchez-Pozo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of nutrition     Volume:  125     ISSN:  0022-3166     ISO Abbreviation:  J. Nutr.     Publication Date:  1995 Jun 
Date Detail:
Created Date:  1995-07-18     Completed Date:  1995-07-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0404243     Medline TA:  J Nutr     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1413-8     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, Faculty of Pharmacy, University of Granada, Spain.
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MeSH Terms
Descriptor/Qualifier:
Adenine Nucleotides / analysis
Adenosine Triphosphate / analysis
Aging / physiology*
Animals
Body Weight / physiology
DNA / analysis
Eating / physiology
Food Deprivation / physiology*
Food, Fortified
Hypoxanthines / analysis
Ileum / drug effects*,  growth & development,  physiology
Intestinal Mucosa / chemistry,  enzymology
Jejunum / drug effects*,  growth & development,  physiology
Lactase
Male
Microvilli / enzymology
Nucleotides / pharmacology*
Proteins / analysis
Random Allocation
Rats
Rats, Wistar
Sucrase / analysis
alpha-Glucosidases / analysis
beta-Galactosidase / analysis
Chemical
Reg. No./Substance:
0/Adenine Nucleotides; 0/Hypoxanthines; 0/Nucleotides; 0/Proteins; 56-65-5/Adenosine Triphosphate; 9007-49-2/DNA; EC 3.2.1.108/Lactase; EC 3.2.1.20/alpha-Glucosidases; EC 3.2.1.23/beta-Galactosidase; EC 3.2.1.48/Sucrase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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