Document Detail

Dietary non-digestible carbohydrates promote L-cell differentiation in the proximal colon of rats.
MedLine Citation:
PMID:  17367575     Owner:  NLM     Status:  MEDLINE    
One of the challenges in type 2 diabetes treatment is to ensure pancreas functionality with gut peptides such as glucagon-like peptide-1 (GLP-1). We have recently shown that the endogenous GLP-1 production is promoted by dietary non-digestible carbohydrates (oligofructose), the higher GLP-1 secretion could participate in the control of obesity and associated disorders. This experimental study was designed to highlight the mechanisms of endogenous increase of GLP-1 following non-digestible carbohydrate feeding. Male Wistar rats were fed a standard diet (70.4 g/100 g total carbohydrates; controls) or the same diet supplemented with oligofructose (10 g/100 g diet) for 4 weeks. GLP-1-producing L-cells of the colon were quantified by immunohistochemistry. GLP-1 was quantified by ELISA, and proglucagon, neurogenin 3 and NeuroD mRNA were measured in the colon by quantitative RT-PCR. The number of GLP-1-expressing cells was doubled in the proximal colon of oligofructose-treated rats, a phenomenon correlated with the increase in proglucagon mRNA and peptide content in the tissue. Moreover, oligofructose increased the number of enteroendocrine L-cells in the proximal colon by a mechanism involving up-regulation of two differentiation factors: neurogenin 3 and NeuroD. It is the first demonstration that nutrients fermented in the gut may promote L-cell differentiation in the proximal colon, a phenomenon contributing to a higher endogenous GLP-1 production. These results suggest a new mechanism by which dietary fibres may lower food intake and fat mass development.
Patrice D Cani; Sophie Hoste; Yves Guiot; Nathalie M Delzenne
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-03-19
Journal Detail:
Title:  The British journal of nutrition     Volume:  98     ISSN:  0007-1145     ISO Abbreviation:  Br. J. Nutr.     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-06-18     Completed Date:  2007-09-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372547     Medline TA:  Br J Nutr     Country:  England    
Other Details:
Languages:  eng     Pagination:  32-7     Citation Subset:  IM    
Unit of Pharmacokinetics, Metabolism, Nutrition and Toxicology, PMNT 73/69, Université catholique de Louvain, Av E Mounier, Brussels, Belgium.
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MeSH Terms
Adipose Tissue / metabolism
Basic Helix-Loop-Helix Transcription Factors / analysis
Body Weight / physiology
Cell Differentiation / physiology
Colon / cytology*,  metabolism
Dietary Carbohydrates / administration & dosage*,  metabolism
Eating / physiology
Enteroendocrine Cells / metabolism,  physiology*
Fermentation / physiology
Glucagon-Like Peptide 1 / biosynthesis*
Nerve Tissue Proteins / analysis
Oligosaccharides / administration & dosage*,  metabolism
Rats, Wistar
Reg. No./Substance:
0/Basic Helix-Loop-Helix Transcription Factors; 0/Dietary Carbohydrates; 0/Nerve Tissue Proteins; 0/Neurog3 protein, rat; 0/Oligosaccharides; 0/oligofructose; 169238-82-8/NeuroD protein; 89750-14-1/Glucagon-Like Peptide 1

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