Document Detail


Dietary n-3 FA modulate long and very long chain FA content, rhodopsin content, and rhodopsin phosphorylation in rat rod outer segment after light exposure.
MedLine Citation:
PMID:  11942475     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A previous study has shown that the long and very long chain FA (VLCFA) content of the rat retina responds to changes in dietary n-6/n-3 ratio of the fat fed (1). The present study tested whether similar changes in these FA are associated with alterations in rhodopsin content and rhodopsin phosphorylation after light treatment. Weanling rats were fed diets containing 20% (w/w, 40% energy) fat with either high (4.8%, w/w) or low (1.2%, w/w) n-3 FA. After 6 wk of feeding, half of the animals in each group were exposed to light for 48 h at 350 lx or were kept in complete darkness. In the rod outer segment, the high n-3 diet treatment increased the level of 20:5n-3 and 22:6n-3 and reduced the levels of 20:4n-6 and 24:4n-6 in PC, PE, and PS. After the feeding of a high n-3 FA diet, total n-3 pentaenoic VLCFA from C24 to C34 increased in PC, whereas the n-6 tetra- and pentaenoic VLCFA decreased. No changes occurred in n-3 hexaenoic VLCFA regardless of the level of 22:6n-3 in the diet. After light exposure, animals fed a high n-3 FA diet showed reduction in 22:6n-3 as well as in n-6 and n-3 VLCFA in PC. FFA and TG fractions contained increased levels of both 20:4n-6 and 22:6n-3 after light exposure. Dark-adapted rhodopsin content and rhodopsin phosphorylation in the rod outer segment of rats fed the low n-3 FA diet were higher than in animals fed a high n-3 FA diet. After light exposure, animals fed the low n-3 FA diet lost more rhodopsin compared to animals fed the high n-3 FA diet, resulting in less phosphorylation of rhodopsin. Results indicate that the FA composition, rhodopsin content, and phosphorylation in visual cells is influenced by the dietary n-3 FA fed as well as by light exposure. The results also imply that 22:6n-3 may not be the precursor for synthesis of hexaenoic VLCFA.
Authors:
Miyoung Suh; Antony A Wierzbicki; M Thomas Clandini
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Lipids     Volume:  37     ISSN:  0024-4201     ISO Abbreviation:  Lipids     Publication Date:  2002 Mar 
Date Detail:
Created Date:  2002-04-10     Completed Date:  2002-10-10     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0060450     Medline TA:  Lipids     Country:  United States    
Other Details:
Languages:  eng     Pagination:  253-60     Citation Subset:  IM    
Affiliation:
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Canada.
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MeSH Terms
Descriptor/Qualifier:
Animals
Fatty Acids / metabolism*
Fatty Acids, Omega-3 / administration & dosage,  pharmacology*
Light*
Male
Phosphorylation
Rats
Rats, Sprague-Dawley
Rhodopsin / metabolism*
Rod Cell Outer Segment / metabolism,  radiation effects*
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Fatty Acids, Omega-3; 9009-81-8/Rhodopsin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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