Document Detail


Dietary (n-3) and (n-6) polyunsaturated fatty acids rapidly modify fatty acid composition and insulin effects in rat adipocytes.
MedLine Citation:
PMID:  9482757     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The influence of dietary (n-3) compared with (n-6) polyunsatured fatty acids (PUFA) on the lipid composition and metabolism of adipocytes was evaluated in rats over a period of 1 week. Isocaloric diets comprised 16.3 g/100 g protein, 53.8 g/100 g carbohydrate and 21.4 g/100 g lipids, the latter containing either (n-3) PUFA (32.4 mol/100 mol) or (n-6) PUFA (37.8 mol/100 mol) but having identical contents of saturated, monounsaturated and total unsaturated fatty acids and identical polyunsaturated to saturated fatty acid ratios and double bond indexes. Despite comparable food intake, significantly smaller body weight increments and adipocyte size were observed in rats of the (n-3) diet group after feeding for 1 wk. Rats fed the (n-3) diet also had significantly lower concentrations of serum triglycerides, cholesterol and insulin compared with those fed the (n-6) diet, although levels of serum glucose and free fatty acids did not differ in the two dietary groups. In the (n-6) diet group, the (n-6) and (n-3) PUFA contents of plasma triglycerides, free fatty acids and phospholipids were 30-60% higher and 60-80% lower, respectively, than in the (n-3) diet group, whereas adipocyte plasma membrane phospholipids showed a significantly higher unsaturated to saturated fatty acid ratio and greater fluidity. Glycerol release in response to noradrenaline was significantly higher in the adipocytes of rats fed the (n-3) diet, whereas the antilipolytic effect of insulin generally did not differ in the two groups. Finally, insulin stimulated the transport of glucose and its incorporation into fatty acids to a lesser extent in adipocytes of (n-3) diet fed rats compared with (n-6) diet fed rats. This reduction in the metabolic effects of insulin in rats fed a (n-3) diet for 1 wk could be related to smaller numbers and a lower binding capacity of the insulin receptors on adipocytes and/or to a lesser degree of phosphorylation of the 95 kDa beta subunit of the receptor. In conclusion, dietary intake for 1 wk of (n-3) rather than (n-6) PUFA is sufficient to induce significant differences in the lipid composition and metabolic responses to insulin of rat adipocytes.
Authors:
M Fickova; P Hubert; G Crémel; C Leray
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of nutrition     Volume:  128     ISSN:  0022-3166     ISO Abbreviation:  J. Nutr.     Publication Date:  1998 Mar 
Date Detail:
Created Date:  1998-04-02     Completed Date:  1998-04-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0404243     Medline TA:  J Nutr     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  512-9     Citation Subset:  IM    
Affiliation:
Institute of Experimental Endocrinology, Slovak Academy of Sciences, 83306 Bratislava, Slovakia.
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MeSH Terms
Descriptor/Qualifier:
Adipocytes / drug effects*,  metabolism*
Animals
Cell Membrane / metabolism
Fatty Acids / metabolism*
Fatty Acids, Omega-3 / pharmacology*
Fatty Acids, Omega-6
Fatty Acids, Unsaturated / pharmacology*
Insulin / metabolism,  pharmacology*
Male
Phosphorylation
Rats
Rats, Wistar
Time Factors
Tyrosine / metabolism
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Fatty Acids, Omega-3; 0/Fatty Acids, Omega-6; 0/Fatty Acids, Unsaturated; 11061-68-0/Insulin; 55520-40-6/Tyrosine

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