Document Detail


Dietary monounsaturated fat activates metabolic pathways for triglyceride-rich lipoproteins that involve apolipoproteins E and C-III.
MedLine Citation:
PMID:  18689361     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Dietary monounsaturated fat (MUFA) and complex carbohydrates have different effects on triglyceride-rich lipoprotein (TRL) metabolism.
OBJECTIVE: We hypothesized that apolipoprotein (apo) E and apo C-III might be involved in these dietary effects because of their crucial role in TRL metabolism.
DESIGN: Twelve adults consumed, for 3 wk each, 2 isocaloric diets: first a carbohydrate-rich diet (48% complex carbohydrate, 8% MUFAs) and then a MUFA-rich diet (31% complex carbohydrate, 24% MUFAs) 12 mo later. The dietary composition of other macronutrients in the 2 diets was similar. Body weight was kept constant. Postprandial apo B kinetic studies using stable-isotope tracers were performed after each dietary intervention. Multiple VLDL, intermediate-density lipoprotein (IDL), and LDL fractions were prepared on the basis of apo E and apo C-III contents.
RESULTS: The MUFA diet increased by approximately 4-6-fold, the secretion of VLDLs and IDLs containing both apo E and apo C-III (E+CIII+) (P < 0.05). These are TRLs that mostly cleared from the circulation and are minor precursors of LDL. The MUFA diet also decreased by 60% (P < 0.05) the secretion of the TRLs without apo E or apo C-III (major precursors of LDL in plasma) and decreased their flux to LDLs. Total LDL flux did not change because the MUFA diet increased the flux to LDL from E-CIII+ TRLs, a process that requires the removal of apo C-III. In addition, the MUFA diet significantly increased the TRL fractional catabolic rate by 50% and doubled the percentage of TRLs that were cleared rather than being converted to LDLs.
CONCLUSION: MUFA intake activates synthetic and rapid catabolic pathways for TRL metabolism that involve apo E and apo C-III and suppresses the metabolism of more slowly metabolized VLDLs and IDLs, which do not contain these apolipoproteins.
Authors:
Chunyu Zheng; Christina Khoo; Jeremy Furtado; Katsunori Ikewaki; Frank M Sacks
Publication Detail:
Type:  Journal Article; Randomized Controlled Trial; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  The American journal of clinical nutrition     Volume:  88     ISSN:  1938-3207     ISO Abbreviation:  Am. J. Clin. Nutr.     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-08-11     Completed Date:  2008-09-12     Revised Date:  2011-10-24    
Medline Journal Info:
Nlm Unique ID:  0376027     Medline TA:  Am J Clin Nutr     Country:  United States    
Other Details:
Languages:  eng     Pagination:  272-81     Citation Subset:  AIM; IM    
Affiliation:
Department of Nutrition, Harvard School of Public Health, Boston, MA, USA.
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MeSH Terms
Descriptor/Qualifier:
Apolipoprotein C-III / blood,  metabolism*
Apolipoproteins E / blood,  metabolism*
Cholesterol, LDL / blood,  metabolism
Cholesterol, VLDL / blood,  metabolism
Cross-Over Studies
Diet*
Dietary Carbohydrates / administration & dosage,  metabolism
Dose-Response Relationship, Drug
Fatty Acids, Monounsaturated / administration & dosage*,  metabolism
Female
Humans
Kinetics
Lipids / blood
Lipoproteins / blood*,  metabolism
Male
Middle Aged
Triglycerides / blood,  metabolism*
Grant Support
ID/Acronym/Agency:
M01 RR002635-140572/RR/NCRR NIH HHS; M01 RR002635-150572/RR/NCRR NIH HHS; M01 RR002635-160572/RR/NCRR NIH HHS; M01 RR002635-170572/RR/NCRR NIH HHS; M01 RR002635-180572/RR/NCRR NIH HHS; M01 RR002635-190572/RR/NCRR NIH HHS; M01 RR002635-200675/RR/NCRR NIH HHS; M01 RR002635-210675/RR/NCRR NIH HHS; M01 RR002635-225334/RR/NCRR NIH HHS; P30 DK040561-13/DK/NIDDK NIH HHS; R01 HL056210-03/HL/NHLBI NIH HHS; R01 HL056210-04/HL/NHLBI NIH HHS; R01 HL056210-05/HL/NHLBI NIH HHS; R01-HL-34980/HL/NHLBI NIH HHS; R01-HL-56210/HL/NHLBI NIH HHS; RR02635/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Apolipoprotein C-III; 0/Apolipoproteins E; 0/Cholesterol, LDL; 0/Cholesterol, VLDL; 0/Dietary Carbohydrates; 0/Fatty Acids, Monounsaturated; 0/Lipids; 0/Lipoproteins; 0/Triglycerides
Comments/Corrections

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