| Dietary mannan oligosaccharide supplementation modulates intestinal microbial ecology and improves gut morphology of rainbow trout, Oncorhynchus mykiss (Walbaum). | |
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MedLine Citation:
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PMID: 19617514 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A study was conducted to investigate the effect of mannan oligosaccharide (MOS) on the gut microbiota and intestinal morphology of rainbow trout under commercial farming conditions. Juvenile (mean initial BW 38.2 +/- 1.7 g) and subadult (111.7 +/- 11.6 g) trout were fed 2 dietary treatments for 111 and 58 d, respectively. The control treatment consisted of a standard commercial diet, and the MOS treatment consisted of the control diet supplemented with 0.2% MOS. Morphology of the anterior and the posterior intestine was examined with light and electron microscopy. Light microscopy demonstrated increased gut absorptive surface area in the subadult MOS group. Additionally, electron microscopy revealed an increase in microvilli length and density in the subadult MOS group compared with the control (P < 0.05). However, no significant improvements were detected in the juvenile group. Culture-based evaluation of the intestinal microbiota showed that MOS significantly reduced (P < 0.05) the viable intestinal bacterial populations (by approximately 2 log scales in all cases). Levels of Aeromonas/Vibrio spp. were significantly decreased (P < 0.05) in the juvenile MOS group (9% of the total microbiota) compared with the juvenile control group (37%). Additionally, analysis of microbial communities was conducted using denaturing gradient gel electrophoresis of PCR-amplified 16S rDNA. The denaturing gradient gel electrophoresis fingerprinting revealed an alteration of bacterial populations; analysis of similarity, similarity percentages, and nonmetric multidimensional scaling analysis showed that MOS reduced species richness and increased similarity of bacterial populations found within the subadult and juvenile groups. The current study shows that MOS modulates intestinal microbial communities, which subsequently improve gut morphology and epithelial brush border. |
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Authors:
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A Dimitroglou; D L Merrifield; R Moate; S J Davies; P Spring; J Sweetman; G Bradley |
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Publication Detail:
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Type: Journal Article Date: 2009-07-17 |
Journal Detail:
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Title: Journal of animal science Volume: 87 ISSN: 1525-3163 ISO Abbreviation: J. Anim. Sci. Publication Date: 2009 Oct |
Date Detail:
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Created Date: 2009-09-18 Completed Date: 2009-12-16 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8003002 Medline TA: J Anim Sci Country: United States |
Other Details:
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Languages: eng Pagination: 3226-34 Citation Subset: IM |
Affiliation:
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School of Biological Sciences, University of Plymouth, Plymouth, Devon, UK . arkadios.dimitroglou@gmail.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Aquaculture Colony Count, Microbial / veterinary DNA, Bacterial / chemistry, genetics Dietary Carbohydrates / pharmacology* Electrophoresis, Polyacrylamide Gel / veterinary Histocytochemistry / veterinary Intestines / metabolism, microbiology*, ultrastructure Mannans / pharmacology* Microscopy, Electron, Scanning / veterinary Microscopy, Electron, Transmission / veterinary* Oligosaccharides / pharmacology* Oncorhynchus mykiss / metabolism*, microbiology Polymerase Chain Reaction / veterinary RNA, Ribosomal, 16S / chemistry, genetics |
| Chemical | |
Reg. No./Substance:
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0/DNA, Bacterial; 0/Dietary Carbohydrates; 0/Mannans; 0/Oligosaccharides; 0/RNA, Ribosomal, 16S |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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