| Dietary manipulation of macrophage phospholipid classes: selective increase of dihomogammalinolenic acid. | |
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MedLine Citation:
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PMID: 3185109 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Because alterations in the dietary content of fatty acids are an important method for modulating macrophage eicosanoid production, we have quantitated the levels of n-6 and n-3 polyunsaturated fatty acids in peritoneal macrophage individual phospholipids from mice fed diets (3 wk) with either safflower oil (SAF), predominantly containing 18:2n-6, borage (BOR) containing 18:2n-6 and 18:3n-6, fish (MFO) containing 20:5n-3 and 22:6n-3, and borage/fish mixture (MIX) containing 18:2n-6, 18:3n-6, 20:5n-3 and 22:6n-3. Dietary n-3 fatty acids were readily incorporated into macrophage phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylserine (PS) and phosphatidylinositol (PI). The increase in n-3 fatty acid levels was accompanied by a decrease in the absolute levels of 18:2n-6, 20:4n-6 and 22:4n-6 in PC, PE and PS. Interestingly, PI 20:4n-6 levels were not significantly lowered (P greater than 0.05) in MIX and MFO macrophages relative to SAF and BOR. These data demonstrate the unique ability of this phospholipid to selectively maintain its 20:4n-6 levels. In BOR and MIX animals, 20:3n-6 levels were significantly increased (P less than 0.05) in all phospholipids relative to SAF and MFO. The combination of borage and fish oils (MIX diet) produced the highest 20:3n-6/20:4n-6 ratio in all phospholipids. These data show that the macrophage eicosanoid precursor levels of 20:3n-6, 20:4n-6 and n-3 acids can be selectively manipulated through the use of specific dietary regimens. This is noteworthy because an increase in phospholipid levels of 20:3n-6 and 20:5n-3, while concomitantly reducing 20:4n-6, may have therapeutic potential in treating inflammatory disorders. |
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Authors:
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R S Chapkin; S D Somers; K L Erickson |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Lipids Volume: 23 ISSN: 0024-4201 ISO Abbreviation: Lipids Publication Date: 1988 Aug |
Date Detail:
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Created Date: 1988-12-07 Completed Date: 1988-12-07 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0060450 Medline TA: Lipids Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 766-70 Citation Subset: IM |
Affiliation:
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Department of Human Anatomy, School of Medicine, University of California, Davis 95616. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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8,11,14-Eicosatrienoic Acid
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biosynthesis* Animals Cells, Cultured Dietary Fats, Unsaturated / pharmacology* Fatty Acids / analysis Fatty Acids, Unsaturated / biosynthesis* Lipids / analysis Macrophages / metabolism* Mice Mice, Inbred C57BL Phospholipids / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Dietary Fats, Unsaturated; 0/Fatty Acids; 0/Fatty Acids, Unsaturated; 0/Lipids; 0/Phospholipids; 7324-41-6/8,11,14-Eicosatrienoic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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