| Dietary manipulation of delta-6-desaturase modifies phospholipid arachidonic acid levels and the urinary excretion of calcium and oxalate in the rat: insight in calcium lithogenesis. | |
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MedLine Citation:
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PMID: 10638699 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An anomalous n-6 polyunsaturated fatty acid composition in plasma and erythrocyte membrane phospholipids, namely increased levels of arachidonic acid (AA), has been reported in calcium nephrolithiasis and has been proposed to play an important role in its pathogenesis. To confirm this, in rats we modified phospholipid AA levels by dietary manipulation of the delta-6-desaturase, the rate-limiting enzyme of the fatty acid biosynthetic pathway, and evaluated the effect on cellular and renal functions predisposing to lithogenesis. Increased AA levels led to conditions at risk for nephrolithiasis: higher oxalate flux and lower sodium cotransport in erythrocytes and a rise in urinary prostaglandin E2, calcium, sodium, and oxalate levels; reduced AA levels reversed these changes. In vitro, in human erythrocytes the incorporation of exogenous AA into membranes increased band 3 protein phosphorylation directly activating the Ser/Thr protein kinase CK1 and induced a parallel raise in band 3-mediated oxalate transport. These findings demonstrate the pivotal role of phospholipid AA in modulating erythrocyte and renal transport of calcium and oxalate. |
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Authors:
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G Gambaro; A Bordoni; S Hrelia; L Bordin; P Biagi; A Semplicini; G Clari; E Manzato; B Baggio |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of laboratory and clinical medicine Volume: 135 ISSN: 0022-2143 ISO Abbreviation: J. Lab. Clin. Med. Publication Date: 2000 Jan |
Date Detail:
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Created Date: 2000-02-01 Completed Date: 2000-02-01 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0375375 Medline TA: J Lab Clin Med Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 89-95 Citation Subset: AIM; IM |
Affiliation:
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Department of Medical and Surgical Sciences, School of Medicine, University of Padova, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anion Exchange Protein 1, Erythrocyte / metabolism Arachidonic Acid / blood*, pharmacology Calcium / urine* Casein Kinase II Casein Kinases Diet* Dinoprostone / urine Erythrocyte Membrane / drug effects, enzymology Erythrocytes / drug effects, metabolism Fatty Acid Desaturases / metabolism* Humans Linoleoyl-CoA Desaturase Liver / enzymology Male Nephrocalcinosis / etiology, metabolism* Oxalates / urine* Phospholipids / blood* Protein Kinases / metabolism Protein-Serine-Threonine Kinases / metabolism Rats Rats, Sprague-Dawley Sodium / urine |
| Chemical | |
Reg. No./Substance:
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0/Anion Exchange Protein 1, Erythrocyte; 0/Oxalates; 0/Phospholipids; 363-24-6/Dinoprostone; 506-32-1/Arachidonic Acid; 7440-23-5/Sodium; 7440-70-2/Calcium; EC 1.14.19.-/Fatty Acid Desaturases; EC 1.14.19.3/Linoleoyl-CoA Desaturase; EC 2.7.-/Protein Kinases; EC 2.7.11.1/Casein Kinase II; EC 2.7.11.1/Casein Kinases; EC 2.7.11.1/Protein-Serine-Threonine Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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