Document Detail

Dietary lipids and sweeteners regulate glucagon-like peptide-2 secretion.
MedLine Citation:
PMID:  23370677     Owner:  NLM     Status:  Publisher    
Glucagon-like peptide-2 (GLP-2) is a potent intestinal growth factor derived from enteroendocrine L-cells. Although food intake is known to increase GLP-2 secretion, its regulatory mechanisms are largely unknown owing to its very short half-life in venules. The aims of this study were to compare the effects of luminal nutrients on the stimulation of GLP-2 secretion in vivo using lymph samples and to clarify the involvement of the sweet taste receptor in this process in vitro. Lymph samples were collected from the thoracic duct after bolus administration of dietary lipids or sweetening agents into the duodenum of rats. Human enteroendocrine NCI-H716 cells were also used to compare the effects of various nutrients on GLP-2 secretion. GLP-2 concentrations were measured by ELISA in vivo and in vitro. GLP-2 secretion was enhanced by polyunsaturated fatty acid (PUFA) and monounsaturated fatty acid (MUFA)-rich dietary oils, dietary carbohydrates, and some kinds of sweeteners in rats; this effect was reproduced in NCI-H716 cells using alpha-linolenic acid (αLA), glucose, and sweeteners. GLP-2 secretion induced by sweetening agents was inhibited by lactisole, a sweetness-antagonizing inhibitor of T1R3. In contrast, lactisole was unable to inhibit GLP-2 secretion induced by αLA alone. Our results suggested that fatty acid- and sweetener-induced GLP-2 secretion may be mediated by 2 different pathways, with the sweet taste receptor involved in the regulation of the latter.
Shingo Sato; Ryota Hokari; Chie Kurihara; Hirokazu Sato; Kazuyuki Narimatsu; Hideaki Hozumi; Toshihide Ueda; Masaaki Higashiyama; Yoshikiyo Okada; Chikako Watanabe; Komoto Shunsuke; Kengo Tomita; Atsushi Kawaguchi; Shigeaki Nagao; Soichiro Miura
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-31
Journal Detail:
Title:  American journal of physiology. Gastrointestinal and liver physiology     Volume:  -     ISSN:  1522-1547     ISO Abbreviation:  Am. J. Physiol. Gastrointest. Liver Physiol.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-2-1     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100901227     Medline TA:  Am J Physiol Gastrointest Liver Physiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
1National Defense Medical College.
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