Document Detail

Dietary lipids influence the activity of delta 5-desaturase and phospholipid fatty acids in rat enterocyte microsomal membranes.
MedLine Citation:
PMID:  9360016     Owner:  NLM     Status:  MEDLINE    
Previous studies have shown that isocaloric modifications in the type of lipids in the diet alter the nutrient uptake and lipid composition of the intestinal brush border membrane. In this study adult rats were fed for 2 weeks isocaloric semisynthetic diets with triglycerides enriched with either saturated (S) or polyunsaturated (P) fatty acids. Enterocyte microsomal membranes (EMMs) were isolated from along the jejunal villus. The activity of delta 5-desaturase was higher in the upper than in the lower portions of the villus, and was greater in P than in S. The activity of delta 9- and delta 6-desaturases did not vary along the villus or with changes in dietary S or P. The two predominant EMM phospholipids were phosphatidylcholine and phosphatidylethanolamine, and these did not vary along the villus or with changes in diet. The major EMM fatty acids in phosphatidylcholine and phosphatidylethanolamine were 16:0, 18:0, 18:2 omega 6, and 20:4 omega 6; none of the individual fatty acids varied along the villus or with diet, although minor changes in fatty acid classes were observed. Thus, alterations in dietary lipids modify the activity of delta 5-desaturase in the EMMs collected from the upper portion of the villus, but this does not result in the expected changes in fatty acids in the EMM phospholipids.
M Keelan; M T Clandinin; A B Thomson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Canadian journal of physiology and pharmacology     Volume:  75     ISSN:  0008-4212     ISO Abbreviation:  Can. J. Physiol. Pharmacol.     Publication Date:  1997 Aug 
Date Detail:
Created Date:  1997-11-20     Completed Date:  1997-11-20     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0372712     Medline TA:  Can J Physiol Pharmacol     Country:  CANADA    
Other Details:
Languages:  eng     Pagination:  1009-14     Citation Subset:  IM    
University of Alberta, Edmonton, Canada.
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MeSH Terms
Dietary Fats / pharmacology*
Fatty Acid Desaturases / metabolism*
Fatty Acids / analysis*,  pharmacology
Fatty Acids, Unsaturated / pharmacology
Intestinal Mucosa / metabolism
Jejunum / drug effects*,  metabolism*
Microsomes / metabolism*
Phospholipids / metabolism*
Rats, Wistar
Reg. No./Substance:
0/Dietary Fats; 0/Fatty Acids; 0/Fatty Acids, Unsaturated; 0/Phospholipids; EC 1.14.19.-/Fatty Acid Desaturases; EC 1.14.99.-/delta-5 fatty acid desaturase

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