Document Detail

Dietary iron status has little effect on expression of ceruloplasmin but alters that of ferritin in rats.
MedLine Citation:
PMID:  11880554     Owner:  NLM     Status:  MEDLINE    
Evidence supports a role for ceruloplasmin (ferroxidase I) in the release of iron to the blood from mammalian cells. However, recent studies with cultured cells have suggested that it has the opposite effect, and that iron deficiency enhances expression of ceruloplasmin. We therefore examined in rats how nutritional iron status would affect expression of ceruloplasmin. Groups of male Sprague-Dawley rats were reared on a low iron, starch-based diet for 6-8 wk; half were supplemented by injection of iron dextran. At killing, hematocrits of deficient rats were half normal. Supplemented rats had normal liver concentrations of ferritin and ferritin iron. No ferritin was detected in the livers of the deficient rats. Northern analysis showed that ferritin L and H mRNAs were present in the deficient livers, but expression was half that of the normal rats. There was also twice as much copper. Levels of circulating ceruloplasmin (measured by rocket immunoelectrophoresis) were not altered by iron deficiency, although p-phenylenediamine oxidase activity and plasma copper were reduced approximately 30%. In repeated studies, no differences in the expression of hepatic ceruloplasmin mRNA were detected. Treatment of rats of both sexes with additional iron (25 mg as iron dextran) 5-14 d before killing increased liver ferritin but did not alter liver ceruloplasmin mRNA expression or levels of circulating ceruloplasmin. We conclude that iron status is not an important factor in the expression of plasma ceruloplasmin made by the liver. However, it does have modest effects on steady-state levels of liver ferritin mRNA.
Tanya Tran; Mubeen Ashraf; LaTrice Jones; Terence Westbrook; Maryam Hazegh-Azam; Maria C Linder
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of nutrition     Volume:  132     ISSN:  0022-3166     ISO Abbreviation:  J. Nutr.     Publication Date:  2002 Mar 
Date Detail:
Created Date:  2002-03-06     Completed Date:  2002-04-09     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0404243     Medline TA:  J Nutr     Country:  United States    
Other Details:
Languages:  eng     Pagination:  351-6     Citation Subset:  IM    
Department of Chemistry and Biochemistry, Institute for Molecular Biology and Nutrition, California State University, Fullerton, CA 92834-6866.
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MeSH Terms
Animal Nutritional Physiological Phenomena*
Blotting, Northern
Ceruloplasmin / analysis,  genetics*
Copper / blood
Ferritins / blood,  genetics*
Gene Expression / drug effects*
Iron, Dietary / administration & dosage*
Liver / chemistry
Nutritional Status*
RNA, Messenger / analysis
Rats, Sprague-Dawley
Grant Support
Reg. No./Substance:
0/Iron, Dietary; 0/RNA, Messenger; 7440-50-8/Copper; 9007-73-2/Ferritins; EC

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