| Dietary iron-deficient anemia induces a variety of metabolic changes and even apoptosis in rat liver: a DNA microarray study. | |
| | |
MedLine Citation:
|
PMID: 20388835 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Anemia can be induced by dietary iron deficiency, as well as by hemorrhagia. It may also be associated with changes in lipid metabolism. However, no global analysis detailing the consequences of iron deficiency in the liver has yet been conducted. Since the liver is a metabolically important organ and also a major iron-storing organ, we performed a comprehensive transcriptome analysis to determine the effects of iron deficiency on hepatic gene expression. Four-week-old rats were fed an iron-deficient diet, approximately 3 ppm iron, ad libitum for 16 days. These rats were compared with similar rats pair-fed a control diet with a normal iron level, 48 ppm iron. The 16-day iron-deficient diet apparently induced anemia. On day 17, the rats were killed under anesthesia, and their livers were dissected for DNA microarray analysis. We identified 600 upregulated and 500 downregulated probe sets that characterized the iron-deficient diet group. In the upregulated probe sets, genes involved in cholesterol, amino acid, and glucose metabolism were significantly enriched, while genes related to lipid metabolism were significantly enriched in the downregulated probe sets. We also found that genes for caspases 3 and 12, which mediate endoplasmic reticulum (ER)-specific apoptosis, were upregulated in the iron-deficient group. Combined, these results suggest that iron deficiency exerts various influences, not only on nutrient metabolism but also on apoptosis, as a consequence of ER stress in the liver. |
| | |
Authors:
|
Asuka Kamei; Yuki Watanabe; Tomoko Ishijima; Mariko Uehara; Soichi Arai; Hisanori Kato; Yuji Nakai; Keiko Abe |
Related Documents
:
|
8950755 - Malaric placentas. a quantitative study and clinico-pathological correlations. 12401305 - Clinical implications of changes in the modern diet: iron intake, absorption and status. 9480645 - Dehalogenation and biodegradation of brominated phenols and benzoic acids under iron-re... 17726315 - Modification of iron status in young overweight/mildly obese women by two dietary inter... 1640845 - Pravastatin lowers serum cholesterol, cholesterol-precursor sterols, fecal steroids, an... 20205925 - Comparative antilipidemic effect of n-acetylcysteine and sesame oil administration in d... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-04-13 |
Journal Detail:
|
Title: Physiological genomics Volume: 42 ISSN: 1531-2267 ISO Abbreviation: Physiol. Genomics Publication Date: 2010 Jul |
Date Detail:
|
Created Date: 2010-07-08 Completed Date: 2010-10-28 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9815683 Medline TA: Physiol Genomics Country: United States |
Other Details:
|
Languages: eng Pagination: 149-56 Citation Subset: IM |
Affiliation:
|
Food Safety and Reliability Project, Kanagawa Academy of Science and Technology, Takatsu-ku, Kawasaki, Kanagawa, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Anemia, Iron-Deficiency
/
genetics,
metabolism* Animals Apoptosis* Diet Iron / blood, metabolism* Lipid Metabolism Liver / metabolism* Male Oligonucleotide Array Sequence Analysis / methods Rats |
| Chemical | |
Reg. No./Substance:
|
7439-89-6/Iron |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Skeletal muscle protein balance in mTOR heterozygous mice in response to inflammation and leucine.
Next Document: Intrauterine Growth Retardation Affects Expression and Epigenetic Characteristics of the Rat Hippoca...