| Dietary glycated protein modulates the colonic microbiota towards a more detrimental composition in ulcerative colitis patients and non-ulcerative colitis subjects. | |
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MedLine Citation:
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PMID: 18399977 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIM: To investigate the effect of native, heated and glycated bovine serum albumin (BSA) on the ulcerative colitis (UC) and non-UC colonic microbiota in vitro. METHODS AND RESULTS: Continuous flow culture (CFC) models of the human colonic microbiota inoculated with faeces from UC and non-UC volunteers were maintained on BSA as growth substrate. Changes in bacterial populations and short-chain fatty acids were determined. UC and non-UC microbiota differed significantly in microbial populations, with elevated numbers of sulfate-reducing bacteria (SRB) and clostridia in the microbiota from UC patients. Compared with native BSA, glycated BSA modulated the gut microbiota of UC patients in vitro towards a more detrimental community structure with significant increases in putatively harmful bacteria (clostridia, bacteroides and SRB; P < 0.009) and decreases in dominant and putatively beneficial bacterial groups (eubacteria and bifidobacteria; P < 0.0004). The levels of beneficial short-chain fatty acids were significantly decreased by heated or glycated BSA, but were increased significantly by native BSA. CONCLUSION: The UC colonic microbiota maintained in CFC was significantly modified by glycated BSA. SIGNIFICANCE AND IMPACT OF THE STUDY: Results suggest that dietary glycated protein may impact upon the composition and activity of the colonic microbiota, an important environmental variable in UC. |
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Authors:
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D J S Mills; K M Tuohy; J Booth; M Buck; M J C Crabbe; G R Gibson; J M Ames |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2008-04-09 |
Journal Detail:
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Title: Journal of applied microbiology Volume: 105 ISSN: 1365-2672 ISO Abbreviation: J. Appl. Microbiol. Publication Date: 2008 Sep |
Date Detail:
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Created Date: 2008-08-21 Completed Date: 2008-12-15 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9706280 Medline TA: J Appl Microbiol Country: England |
Other Details:
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Languages: eng Pagination: 706-14 Citation Subset: IM |
Affiliation:
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School of Chemistry, Food Biosciences and Pharmacy, The University of Reading, Whiteknights, Reading, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Analysis of Variance Animals Bacteria / drug effects*, metabolism Bacteriological Techniques Bacteroides / drug effects, physiology Case-Control Studies Cattle Clostridium / drug effects, physiology Colitis, Ulcerative / microbiology* Colon / microbiology* Colony Count, Microbial / methods Dietary Proteins / pharmacology* Digestion Fatty Acids, Volatile / metabolism Feces / microbiology Female Food* Hot Temperature Humans In Situ Hybridization, Fluorescence Male Middle Aged Ribotyping Serum Albumin / pharmacology* Serum Albumin, Bovine / pharmacology Sulfates / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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//Wellcome Trust |
| Chemical | |
Reg. No./Substance:
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0/Dietary Proteins; 0/Fatty Acids, Volatile; 0/Serum Albumin; 0/Serum Albumin, Bovine; 0/Sulfates; 0/glycosylated serum albumin |
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