Document Detail

Dietary glycated protein modulates the colonic microbiota towards a more detrimental composition in ulcerative colitis patients and non-ulcerative colitis subjects.
MedLine Citation:
PMID:  18399977     Owner:  NLM     Status:  MEDLINE    
AIM: To investigate the effect of native, heated and glycated bovine serum albumin (BSA) on the ulcerative colitis (UC) and non-UC colonic microbiota in vitro. METHODS AND RESULTS: Continuous flow culture (CFC) models of the human colonic microbiota inoculated with faeces from UC and non-UC volunteers were maintained on BSA as growth substrate. Changes in bacterial populations and short-chain fatty acids were determined. UC and non-UC microbiota differed significantly in microbial populations, with elevated numbers of sulfate-reducing bacteria (SRB) and clostridia in the microbiota from UC patients. Compared with native BSA, glycated BSA modulated the gut microbiota of UC patients in vitro towards a more detrimental community structure with significant increases in putatively harmful bacteria (clostridia, bacteroides and SRB; P < 0.009) and decreases in dominant and putatively beneficial bacterial groups (eubacteria and bifidobacteria; P < 0.0004). The levels of beneficial short-chain fatty acids were significantly decreased by heated or glycated BSA, but were increased significantly by native BSA. CONCLUSION: The UC colonic microbiota maintained in CFC was significantly modified by glycated BSA. SIGNIFICANCE AND IMPACT OF THE STUDY: Results suggest that dietary glycated protein may impact upon the composition and activity of the colonic microbiota, an important environmental variable in UC.
D J S Mills; K M Tuohy; J Booth; M Buck; M J C Crabbe; G R Gibson; J M Ames
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-04-09
Journal Detail:
Title:  Journal of applied microbiology     Volume:  105     ISSN:  1365-2672     ISO Abbreviation:  J. Appl. Microbiol.     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2008-08-21     Completed Date:  2008-12-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9706280     Medline TA:  J Appl Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  706-14     Citation Subset:  IM    
School of Chemistry, Food Biosciences and Pharmacy, The University of Reading, Whiteknights, Reading, UK.
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MeSH Terms
Analysis of Variance
Bacteria / drug effects*,  metabolism
Bacteriological Techniques
Bacteroides / drug effects,  physiology
Case-Control Studies
Clostridium / drug effects,  physiology
Colitis, Ulcerative / microbiology*
Colon / microbiology*
Colony Count, Microbial / methods
Dietary Proteins / pharmacology*
Fatty Acids, Volatile / metabolism
Feces / microbiology
Hot Temperature
In Situ Hybridization, Fluorescence
Middle Aged
Serum Albumin / pharmacology*
Serum Albumin, Bovine / pharmacology
Sulfates / metabolism
Grant Support
//Wellcome Trust
Reg. No./Substance:
0/Dietary Proteins; 0/Fatty Acids, Volatile; 0/Serum Albumin; 0/Serum Albumin, Bovine; 0/Sulfates; 0/glycosylated serum albumin

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