| Dietary glutamine and oral antibiotics each improve indexes of gut barrier function in rat short bowel syndrome. | |
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MedLine Citation:
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PMID: 19095767 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Short bowel syndrome (SBS) is associated with gut barrier dysfunction. We examined effects of dietary glutamine (GLN) or oral antibiotics (ABX) on indexes of gut barrier function in a rat model of SBS. Adult rats underwent a 60% distal small bowel + proximal colonic resection (RX) or bowel transection (TX; control). Rats were pair fed diets with or without l-GLN for 20 days after operation. Oral ABX (neomycin, metronidazole, and polymyxin B) were given in some RX rats fed control diet. Stool secretory immunoglobulin A (sIgA) was measured serially. On day 21, mesenteric lymph nodes (MLN) were cultured for gram-negative bacteria. IgA-positive plasma cells in jejunum, stool levels of flagellin- and lipopolysaccharide (LPS)-specific sIgA, and serum total, anti-flagellin- and anti-LPS IgG levels were determined. RX caused gram-negative bacterial translocation to MLN, increased serum total and anti-LPS IgG and increased stool total sIgA. After RX, dietary GLN tended to blunt bacterial translocation to MLN (-29%, P = NS) and significantly decreased anti-LPS IgG levels in serum, increased both stool and jejunal mucosal sIgA and increased stool anti-LPS-specific IgA. Oral ABX eliminated RX-induced bacterial translocation, significantly decreased total and anti-LPS IgG levels in serum, significantly decreased stool total IgA and increased stool LPS-specific IgA. Partial small bowel-colonic resection in rats is associated with gram-negative bacterial translocation from the gut and a concomitant adaptive immune response to LPS. These indexes of gut barrier dysfunction are ameliorated or blunted by administration of dietary GLN or oral ABX, respectively. Dietary GLN upregulates small bowel sIgA in this model. |
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Authors:
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Junqiang Tian; Li Hao; Prakash Chandra; Dean P Jones; Ifor R Willams; Andrew T Gewirtz; Thomas R Ziegler |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2008-12-18 |
Journal Detail:
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Title: American journal of physiology. Gastrointestinal and liver physiology Volume: 296 ISSN: 0193-1857 ISO Abbreviation: Am. J. Physiol. Gastrointest. Liver Physiol. Publication Date: 2009 Feb |
Date Detail:
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Created Date: 2009-01-27 Completed Date: 2009-03-13 Revised Date: 2011-05-10 |
Medline Journal Info:
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Nlm Unique ID: 100901227 Medline TA: Am J Physiol Gastrointest Liver Physiol Country: United States |
Other Details:
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Languages: eng Pagination: G348-55 Citation Subset: IM |
Affiliation:
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Department of Medicine, Emory University, Atlanta, GA 30322, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Administration, Oral Animals Anti-Bacterial Agents / administration & dosage* Antibodies, Bacterial / blood Bacterial Translocation / drug effects* Body Weight Cells, Cultured Dietary Supplements* Disease Models, Animal Drug Therapy, Combination Eating Feces / chemistry Flagellin / metabolism Glutamine / administration & dosage* Immunoglobulin A, Secretory / metabolism Intestines / drug effects*, immunology, microbiology, surgery Lymph Nodes / drug effects, microbiology Male Membrane Proteins / metabolism Metronidazole / administration & dosage Neomycin / administration & dosage Phosphoproteins / metabolism Plasma Cells / drug effects, immunology Polymyxin B / administration & dosage Rats Rats, Sprague-Dawley Short Bowel Syndrome / drug therapy*, immunology, microbiology Tight Junctions / drug effects, metabolism Time Factors |
| Grant Support | |
ID/Acronym/Agency:
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K24 RR023356-02/RR/NCRR NIH HHS; K24 RR023356-05/RR/NCRR NIH HHS; R01 DK055850-05/DK/NIDDK NIH HHS; R01 DK061417/DK/NIDDK NIH HHS; R01 DK55850/DK/NIDDK NIH HHS; R01 ES011195/ES/NIEHS NIH HHS; R01DK064730/DK/NIDDK NIH HHS; R24 DK064399/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Anti-Bacterial Agents; 0/Antibodies, Bacterial; 0/Immunoglobulin A, Secretory; 0/Membrane Proteins; 0/Phosphoproteins; 0/occludin; 0/zonula occludens-1 protein; 12777-81-0/Flagellin; 1404-04-2/Neomycin; 1404-26-8/Polymyxin B; 443-48-1/Metronidazole; 56-85-9/Glutamine |
| Comments/Corrections | |
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