Document Detail


Dietary gamma-linolenic acid in the form of borage oil causes less body fat accumulation accompanying an increase in uncoupling protein 1 mRNA level in brown adipose tissue.
MedLine Citation:
PMID:  11079375     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Rats were fed a low-fat diet containing 2% safflower oil or 20% fat diets containing either safflower oil rich in linoleic acid, borage oil containing 25% gamma (gamma)-linolenic acid or enzymatically prepared gamma-linolenic acid enriched borage oil containing 47% gamma-linolenic acid for 14 days. Energy intake and growth of animals were the same among groups. A high safflower oil diet compared with a low-fat diet caused significant increases in both epididymal and perirenal white adipose tissue weights. However, high-fat diets rich in gamma-linolenic acid failed to do so. Compared with a low-fat diet, all the high-fat diets increased mRNA levels of uncoupling protein 1 and lipoprotein lipase in brown adipose tissue. The extents of the increase were greater with high-fat diets rich in gamma-linolenic acid. Various high-fat diets, compared with a low-fat diet, decreased glucose transporter 4 mRNA in white adipose tissue to the same levels. The amount and types of dietary fat did not affect the leptin mRNA level in epididymal white adipose tissue. However, a high safflower oil diet, but not high-fat diets rich in gamma-linolenic acid relative to a low-fat diet, increased perirenal white adipose tissue leptin mRNA levels. All high-fat diets, relative to a low-fat diet, increased the hepatic mitochondrial fatty acid oxidation rate and fatty acid oxidation enzyme mRNA abundances to the same levels. High-fat diets also increased these parameters in the peroxisomal pathway, and the increases were greater with high-fat diets rich in gamma-linolenic acid. The physiological activity in increasing brown adipose tissue gene expression and peroxisomal fatty acid oxidation was similar between the two types of borage oil differing in gamma-linolenic acid content. It was suggested that dietary gamma-linolenic acid attenuates body fat accumulation through the increase in gene expressions of uncoupling protein 1 in brown adipose tissue. An increase in hepatic peroxisomal fatty acid oxidation may also contribute to the physiological activity of gamma-linolenic acid in decreasing body fat mass.
Authors:
Y Takahashi; T Ide; H Fujita
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology     Volume:  127     ISSN:  1096-4959     ISO Abbreviation:  Comp. Biochem. Physiol. B, Biochem. Mol. Biol.     Publication Date:  2000 Oct 
Date Detail:
Created Date:  2001-02-05     Completed Date:  2001-03-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9516061     Medline TA:  Comp Biochem Physiol B Biochem Mol Biol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  213-22     Citation Subset:  IM    
Affiliation:
Laboratory of Nutrition Biochemistry, National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries, Tsukuba Science City, Ibaraki, Japan. youkot@nfri.affrc.go.jp
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MeSH Terms
Descriptor/Qualifier:
Adipose Tissue / metabolism
Adipose Tissue, Brown / drug effects*,  metabolism
Animals
Carrier Proteins / biosynthesis*
Diet
Diet, Fat-Restricted
Fatty Acids / metabolism
Ion Channels
Leptin / metabolism
Lipoprotein Lipase / metabolism
Liver / enzymology
Male
Membrane Proteins / biosynthesis*
Mitochondria / metabolism
Mitochondrial Proteins
Monosaccharide Transport Proteins / metabolism
Oxygen / metabolism
Palmitoyl Coenzyme A / metabolism
Peroxisomes / metabolism
Plant Oils / pharmacology*
RNA, Messenger / metabolism*
Rats
Safflower Oil / pharmacology
gamma-Linolenic Acid / pharmacology*
Chemical
Reg. No./Substance:
0/Carrier Proteins; 0/Fatty Acids; 0/Ion Channels; 0/Leptin; 0/Membrane Proteins; 0/Mitochondrial Proteins; 0/Monosaccharide Transport Proteins; 0/Plant Oils; 0/RNA, Messenger; 0/borage oil; 0/mitochondrial uncoupling protein; 1763-10-6/Palmitoyl Coenzyme A; 506-26-3/gamma-Linolenic Acid; 7782-44-7/Oxygen; 8001-23-8/Safflower Oil; EC 3.1.1.34/Lipoprotein Lipase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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