| Dietary fructose exacerbates hepatocellular injury when incorporated into a methionine-choline-deficient diet. | |
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MedLine Citation:
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PMID: 20536716 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Methionine-choline-deficient (MCD) diets cause steatohepatitis in rodents and are used to model fatty liver disease in human beings. Recent studies have identified sucrose as a major contributor to MCD-related liver disease through its ability to promote hepatic de novo lipogenesis. AIMS: To determine whether glucose and fructose, the two constitutents of sucrose, differ in their capacity to provoke steatohepatitis when incorporated individually into MCD formulas. MATERIALS & METHODS: MCD and control formulas prepared with either glucose or fructose as the sole source of carbohydrate were fed to mice for 21 days. Liver injury was assessed biochemically and histologically together with hepatic gene expression and fatty acid analysis. RESULTS: Mice fed MCD formulas developed similar degrees of hepatic steatosis whether they contained glucose or fructose. By contrast, mice fed MCD-fructose developed significantly more hepatocellular injury than mice fed MCD-glucose, judged by histology, apoptosis staining and serum alanine aminotransferase. Liver injury in MCD-fructose mice coincided with an exaggerated rise in the ratio of long-chain saturated to unsaturated fatty acids in the liver. Notably, hepatic inflammation was not enhanced in mice fed MCD-fructose, correlating instead with hepatic lipid peroxidation, which was equivalent in the two MCD groups. DISCUSSION: Fructose is more cytotoxic than glucose when used as the source of carbohydrate in MCD formulas. CONCLUSION: The data suggest the enhanced cytotoxicity of fructose in the MCD model is related to its ability to stimulate de novo lipogenesis, which yields harmful long-chain saturated fatty acids. |
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Authors:
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Michael K Pickens; Hisanobu Ogata; Russell K Soon; James P Grenert; Jacquelyn J Maher |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-06-08 |
Journal Detail:
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Title: Liver international : official journal of the International Association for the Study of the Liver Volume: 30 ISSN: 1478-3231 ISO Abbreviation: Liver Int. Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-08-16 Completed Date: 2010-12-20 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101160857 Medline TA: Liver Int Country: England |
Other Details:
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Languages: eng Pagination: 1229-39 Citation Subset: IM |
Affiliation:
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Department of Pediatrics, University of California, San Francisco, CA, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Alanine Transaminase
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blood Analysis of Variance Animals Blood Glucose Cholesterol / blood Choline Deficiency Diet / adverse effects* Dietary Sucrose / adverse effects* Fatty Liver / etiology*, pathology Fructose / adverse effects* Gene Expression Profiling Gene Expression Regulation / drug effects* Lipid Peroxidation / physiology Lipogenesis / drug effects Liver / metabolism, pathology* Male Methionine / deficiency Mice Mice, Mutant Strains Reverse Transcriptase Polymerase Chain Reaction Triglycerides / blood |
| Grant Support | |
ID/Acronym/Agency:
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P30 DK026743/DK/NIDDK NIH HHS; R01 DK068450/DK/NIDDK NIH HHS; T32 DK007762/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Blood Glucose; 0/Dietary Sucrose; 0/Triglycerides; 30237-26-4/Fructose; 57-88-5/Cholesterol; 63-68-3/Methionine; EC 2.6.1.2/Alanine Transaminase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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