Document Detail


Dietary freeze-dried black raspberry's effect on cellular antioxidant status during reflux-induced esophagitis in rats.
MedLine Citation:
PMID:  20538426     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
INTRODUCTION: Esophageal cancer consists of two distinct types, esophageal adenocarcinoma (EAC) and squamous cell carcinoma, both of which differ significantly in their etiology. Freeze-dried black raspberry (BRB) has been consistent in its ability to modulate the biomarkers and reduce the incidence of carcinogen-induced squamous cell carcinoma in rats. In our previous studies in the esophagoduodenal anastomosis (EDA) model, we have shown that the early modulation of manganese superoxide dismutase (MnSOD) significantly correlates with the development of reflux-induced EAC in rats. In this study we looked at the short-term effects of a BRB-supplemented diet on the modulation of antioxidant enzymes in reflux-induced esophagitis.
METHODS: Male SD rats (8 wk old; n = 3-5) were randomized into three groups--sham-operated, fed control AIN-93M diet (SH-CD), EDA operated and fed either control diet (EDA-CD) or 2.5% (w/w) BRB diet (EDA-BRB). The effect of both reflux and dietary supplementation was analyzed 2 and 4 wk after EDA surgery.
RESULTS: Animals in the EDA groups had significantly lower weight gain and diet intake compared to SH-CD (P < 0.05). The sham-operated animals received an average esophagitis score of 0.1 ± 0.1; this increased significantly in EDA-CD animals to 1.8 ± 0.14 (P < 0.001 versus SH-CD) and in EDA-BRB group to 1.7 ± 0.06 (P < 0.001 versus SH-CD), with BE changes also present. However, dietary supplementation of BRB did not alter or ameliorate the grade of esophagitis or the induction of BE. BRB diet caused a 43% increase in MnSOD levels compared to EDA-CD (0.73 ± 0.16; P = 0.09); however, this effect was not statistically significant and at 4 wk, EDA-CD (0.58 ± 0.12) showed an increase in MnSOD expression compared to SH-CD (0.34 ± 0.01).
CONCLUSIONS: In conclusion, our data suggest that dietary BRB does not increase the levels of cellular antioxidant enzymes or reduce the levels of lipid peroxidation compared to a control diet, in a short-term study of gastroesophageal reflux induction in the EDA animal model. However, it remains to be tested whether this is indicative of its ineffectiveness to inhibit reflux-induced EAC incidence over the long term.
Authors:
Harini S Aiyer; Yan Li; Qiao Hong Liu; Nathaniel Reuter; Robert C G Martin
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Publication Detail:
Type:  Journal Article     Date:  2010-06-11
Journal Detail:
Title:  Nutrition (Burbank, Los Angeles County, Calif.)     Volume:  27     ISSN:  1873-1244     ISO Abbreviation:  Nutrition     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-01-10     Completed Date:  2011-04-18     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  8802712     Medline TA:  Nutrition     Country:  United States    
Other Details:
Languages:  eng     Pagination:  182-7     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier Inc. All rights reserved.
Affiliation:
Department of Surgery, Division of Surgical Oncology, University of Louisville School of Medicine, Louisville, Kentucky, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antioxidants / pharmacology
Diet*
Esophagitis, Peptic / drug therapy*,  etiology*,  pathology
Freeze Drying
Fruit / chemistry
Gastroesophageal Reflux / complications*
Lipid Peroxidation
Male
Phytotherapy*
Random Allocation
Rats
Rats, Sprague-Dawley
Rosaceae / chemistry*
Superoxide Dismutase / metabolism
Grant Support
ID/Acronym/Agency:
R03 CA137801-01/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Antioxidants; EC 1.15.1.1/Superoxide Dismutase
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