| Dietary folate and selenium affect dimethylhydrazine-induced aberrant crypt formation, global DNA methylation and one-carbon metabolism in rats. | |
| | |
MedLine Citation:
|
PMID: 12949386 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Several observations suggest a role for DNA methylation in cancer pathogenesis. Although both selenium and folate deficiency have been shown to cause global DNA hypomethylation and increased cancer susceptibility, the nutrients have different effects on one-carbon metabolism. Thus, the purpose of this study was to investigate the interactive effects of dietary selenium and folate. Weanling, Fischer-344 rats (n = 23/diet) were fed diets containing 0 or 2.0 mg selenium (as selenite)/kg and 0 or 2.0 mg folate/kg in a 2 x 2 factorial design. After 3 and 4 wk of a 12-wk experiment, 19 rats/diet were injected intraperitoneally with dimethylhydrazine (DMH, 25 mg/kg) and 4 rats/diet were administered saline. Selenium deficiency decreased (P < 0.05) colonic DNA methylation and the activities of liver DNA methyltransferase and betaine homocysteine methyltransferase and increased plasma glutathione concentrations. Folate deficiency increased (P < 0.05) the number of aberrant crypts per aberrant crypt foci, the concentration of colonic S-adenosylhomocysteine and the activity of liver cystathionine synthase. Selenium and folate interacted (P < 0.0001) to influence one-carbon metabolism and cancer susceptibility such that the number of aberrant crypts and the concentrations of plasma homocysteine and liver S-adenosylhomocysteine were the highest and the concentrations of plasma folate and liver S-adenosylmethionine and the activity of liver methionine synthase were the lowest in rats fed folate-deficient diets and supplemental selenium. These results suggest that selenium deprivation ameliorates some of the effects of folate deficiency, probably by shunting the buildup of homocysteine (as a result of folate deficiency) to glutathione. |
| | |
Authors:
|
Cindy D Davis; Eric O Uthus |
Related Documents
:
|
17287146 - Rodent brain and heart catecholamine levels are altered by different models of copper d... 2484556 - Zinc deficiency is associated with suppression of colonocyte proliferation in the dista... 22123126 - Metabolic fate of sipoglitazar, a novel oral ppar agonist with activities for ppar-γ, ... 3976746 - Intrauterine growth retardation induced by thiamine deficiency and pyrithiamine during ... 12458186 - Sex-dependent pharmacokinetics of s(-)-hydroxyhexamide, a pharmacologically active meta... 12778186 - Bioassay of reserpine for possible carcinogenicity (cas no. 50-55-5). |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: The Journal of nutrition Volume: 133 ISSN: 0022-3166 ISO Abbreviation: J. Nutr. Publication Date: 2003 Sep |
Date Detail:
|
Created Date: 2003-09-01 Completed Date: 2003-10-28 Revised Date: 2003-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 0404243 Medline TA: J Nutr Country: United States |
Other Details:
|
Languages: eng Pagination: 2907-14 Citation Subset: IM |
Affiliation:
|
US Department of Agriculture, Grand Forks Human Nutrition Research Center, Grand Forks, ND 58202-9034, USA. davisci@mail.nih.gov |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase
/
metabolism Animals Antioxidants / administration & dosage* Carbon / metabolism* Carcinogens / administration & dosage* Colon / drug effects*, metabolism, pathology* Cystathionine beta-Synthase / metabolism DNA (Cytosine-5-)-Methyltransferase / metabolism DNA Methylation Deficiency Diseases / genetics, metabolism, pathology Diet Dimethylhydrazines / administration & dosage* Disease Susceptibility Drug Interactions Folic Acid / administration & dosage*, blood Glutathione / blood Homocysteine / blood Injections, Intraperitoneal Liver / enzymology, metabolism Male Rats Rats, Inbred F344 S-Adenosylhomocysteine / metabolism Selenium / administration & dosage*, deficiency |
| Chemical | |
Reg. No./Substance:
|
0/Antioxidants; 0/Carcinogens; 0/Dimethylhydrazines; 454-28-4/Homocysteine; 59-30-3/Folic Acid; 70-18-8/Glutathione; 7440-44-0/Carbon; 7782-49-2/Selenium; 979-92-0/S-Adenosylhomocysteine; EC 2.1.1.13/5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase; EC 2.1.1.37/DNA (Cytosine-5-)-Methyltransferase; EC 4.2.1.22/Cystathionine beta-Synthase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Diallyl disulfide induces ERK phosphorylation and alters gene expression profiles in human colon tum...
Next Document: Nutritional status of infants and young children and characteristics of their diets.