Document Detail

Dietary fish oil promotes positive inotropy of ouabain in the rat heart.
MedLine Citation:
PMID:  10600848     Owner:  NLM     Status:  MEDLINE    
We tested the hypothesis that a fish oil (FO) diet promotes positive inotropy of ouabain without increased toxicity. For 2 mo, two groups of adult male rats were fed 1) a regular food diet supplemented with dietary long-chain polyunsaturated fatty acid from FO or 2) a regular food diet (control). The responsiveness to ouabain was evaluated for the two groups in Langendorff-perfused hearts, by (31)P nuclear magnetic resonance spectroscopy, and on purified membrane-bound Na-K-ATPase. The maximum positive inotropy achieved with ouabain was nearly two times higher in the FO than in the control group and was not associated with significant changes in energetics. Alteration of function and energetic metabolism and inhibition of Na-K-ATPase in response to 3 x 10(-4) M ouabain were delayed in the FO group. This study demonstrates that dietary FO, by a cardiac membrane incorporation of n-3 polyunsaturated fatty acid, promotes positive inotropy of ouabain without toxicity and changes in cardiac metabolism.
J M Maixent; A Gerbi; O Barbey; C Lan; I Jamme; H Burnet; A Nouvelot; S Lévy; P J Cozzone; M Bernard
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The American journal of physiology     Volume:  277     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1999 Dec 
Date Detail:
Created Date:  2000-01-19     Completed Date:  2000-01-19     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  H2290-7     Citation Subset:  IM    
Laboratoire de Recherche Cardiologique, Unité Mixte de Recherche 6612, Centre National de la Recherche Scientifique (CNRS), France.
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MeSH Terms
Cell Membrane / enzymology
Dietary Fats, Unsaturated / pharmacology*
Dietary Supplements
Fish Oils / pharmacology*
Magnetic Resonance Spectroscopy
Myocardial Contraction / drug effects*,  physiology
Myocardium / enzymology
Ouabain / pharmacology*
Rats, Wistar
Sodium-Potassium-Exchanging ATPase / metabolism
Reg. No./Substance:
0/Dietary Fats, Unsaturated; 0/Fish Oils; 630-60-4/Ouabain; EC ATPase

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