Document Detail

Dietary fish oil dose- and time-response effects on cardiac phospholipid fatty acid composition.
MedLine Citation:
PMID:  15691017     Owner:  NLM     Status:  MEDLINE    
Fish consumption is associated with reduced cardiovascular mortality, and elevated myocardial long-chain n-3 polyunsaturated FA (PUFA) content is implicated in this cardioprotection. This study examined the dose and time responses for incorporation of n-3 PUFA into cellular membranes in rats fed fish oil (FO)-containing diets. For the time course study, rats were fed a 10% FO diet for periods ranging from 0 to 42 d, after which myocardial and erythrocyte membrane fatty acid composition was determined. For the dose response study, rats (n = 3) were fed 0, 1.25, 2.5, 5, or 10% FO for 4 wk, with myocardial, erythrocyte, and skeletal muscle membrane FA determined. Myocardial DHA (22:6n-3) levels doubled in 2 d, stabilizing at levels approximately 200% higher than control after 28 d feeding with 10% FO. By comparison, DHA levels doubled after 4 wk of 1.25% FO feeding. In myocardium and skeletal muscle, EPA (20:5n-3) levels remained low, but in erythrocytes EPA levels reached 50% of DHA levels. The n-3 PUFA were incorporated at the expense of n-6 PUFA in myocardium and skeletal muscle, whereas erythrocytes maintained arachidonic acid levels, and total n-3 PUFA incorporation was lower. This study shows that low doses of FO produce marked changes in myocardial DHA levels; maximal incorporation takes up to 28 d to occur; and while erythrocytes are a good indicator of tissue n-3 incorporation in stable diets, they vary greatly in their time course and pattern of incorporation.
Alice J Owen; Beata A Peter-Przyborowska; Andrew J Hoy; Peter L McLennan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Lipids     Volume:  39     ISSN:  0024-4201     ISO Abbreviation:  Lipids     Publication Date:  2004 Oct 
Date Detail:
Created Date:  2005-02-04     Completed Date:  2005-06-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0060450     Medline TA:  Lipids     Country:  United States    
Other Details:
Languages:  eng     Pagination:  955-61     Citation Subset:  IM    
Smart Foods Center, Department of Biomedical Science, University of Wollongong, Wollongong, NSW 2522, Australia.
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MeSH Terms
Cell Membrane / chemistry,  metabolism
Dose-Response Relationship, Drug
Erythrocytes / chemistry,  metabolism
Fatty Acids / analysis*
Fatty Acids, Omega-3 / administration & dosage,  metabolism,  pharmacology
Fish Oils / administration & dosage,  metabolism,  pharmacology*
Muscle, Skeletal / chemistry,  metabolism
Myocardium / chemistry*,  metabolism
Phospholipids / chemistry*
Rats, Wistar
Reg. No./Substance:
0/Fatty Acids; 0/Fatty Acids, Omega-3; 0/Fish Oils; 0/Phospholipids

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