| Dietary fat decreases intestinal levels of the anorectic lipids through a fat sensor. | |
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MedLine Citation:
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PMID: 20959516 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study was undertaken to investigate the link between dietary fat content and intestinal levels of anorectic N-acylethanolamines (NAEs), including oleoylethanolamide (OEA), palmitoylethanolamide (PEA), and linoleoylethanolamide (LEA). Male rats were fed high-fat diets (HFDs) with variable percentages of fat [20-45% of total energy (E%)] for 1-7 d; afterward, the jejunums were isolated, and jejunal NAE levels were measured by liquid-chromatography mass spectrometry. Enzyme activities and mRNA expression levels were measured for two synthesizing enzymes, N-acylphosphatidylethanolamine-specific phospholipase D (NAPE-PLD) and glycerophosphodiesterase (GDE1), and one degrading enzyme, fatty acid amide hydrolase (FAAH). We found a dose-response relation between the quantity/percentage of dietary fat, irrespective of the energy density, and the reduction of intestinal levels of OEA, PEA, and LEA. The reductions were present after 1 d of 45E% HFD. LEA, the major NAE species, was shown to have an anorectic potency slightly less than that of OEA but higher than PEA. Regulation at the enzyme level seems not to explain the changes in NAE levels. The results suggest the presence of a fat sensor, mediating the reduced intestinal NAE levels. The intestinal NAE levels are reduced in a dose- and time-dependent manner in response to dietary fat intake, and this may contribute to the well-known hyperphagic effect of HFDs. |
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Authors:
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Thi Ai Diep; Andreas Nygaard Madsen; Birgitte Holst; Martin Mørch Kristiansen; Niels Wellner; Steen Honoré Hansen; Harald Severin Hansen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-10-19 |
Journal Detail:
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Title: FASEB journal : official publication of the Federation of American Societies for Experimental Biology Volume: 25 ISSN: 1530-6860 ISO Abbreviation: FASEB J. Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-02-01 Completed Date: 2011-04-04 Revised Date: 2012-02-15 |
Medline Journal Info:
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Nlm Unique ID: 8804484 Medline TA: FASEB J Country: United States |
Other Details:
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Languages: eng Pagination: 765-74 Citation Subset: IM |
Affiliation:
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Department of Pharmacology and Pharmacotheraphy, Faculty of Pharmaceutical Sciences, University of Copenhagen, Copenhagen, Denmark. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Dietary Fats / pharmacology* Dose-Response Relationship, Drug Eating Gene Expression Regulation, Enzymologic / physiology Intestines / drug effects*, enzymology, metabolism* Linoleic Acids / pharmacology Lipids / physiology* Male Polyunsaturated Alkamides / pharmacology RNA, Messenger Random Allocation Rats Rats, Sprague-Dawley |
| Chemical | |
Reg. No./Substance:
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0/Dietary Fats; 0/Linoleic Acids; 0/Lipids; 0/Polyunsaturated Alkamides; 0/RNA, Messenger; 68171-52-8/linoleoyl ethanolamide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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