Document Detail

Dietary lutein/zeaxanthin decreases ultraviolet B-induced epidermal hyperproliferation and acute inflammation in hairless mice.
MedLine Citation:
PMID:  12880433     Owner:  NLM     Status:  MEDLINE    
Lutein and zeaxanthin are carotenoids found in green leafy vegetables with interesting antioxidant properties. They are present in high concentrations in the fovea centralis of the human retina and their role in the prevention of age-related macula degeneration has been reported. We have investigated the effect of orally administered lutein and zeaxanthin in the cutaneous response to ultraviolet B irradiation. Female hairless SKh-1 mice receiving 0.4% and 0.04% lutein plus zeaxanthin-enriched diet for 2 wk were exposed to single doses of ultraviolet B radiation. Skin biopsies were taken at 24 and 48 h after irradiation and analyzed for the presence of apoptotic cells, proliferating cells, and expression of proliferating cell nuclear antigen. Our results show a clear ultraviolet-induced dose-dependent inflammatory response. Orally administered 0.4% lutein and zeaxanthin decreased significantly the edematous cutaneous response (p<0.01) as determined by the reduction of the UVB-induced increase of ear bifold thickening. Additionally, dietary carotenoids were efficient in reducing the ultraviolet B-induced increases in the percentage of proliferating cell nuclear antigen (p<0.05), bromodeoxyuridine (p<0.05), and terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end-labeling-positive cells (p<0.01). These data demonstrate that oral supplementation of lutein and zeaxanthin diminishes the effects of ultraviolet B irradiation by reducing acute inflammatory responses and ultraviolet-induced hyperproliferative rebound.
Salvador González; Susi Astner; Wu An; David Goukassian; Madhu A Pathak
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of investigative dermatology     Volume:  121     ISSN:  0022-202X     ISO Abbreviation:  J. Invest. Dermatol.     Publication Date:  2003 Aug 
Date Detail:
Created Date:  2003-07-25     Completed Date:  2003-09-16     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0426720     Medline TA:  J Invest Dermatol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  399-405     Citation Subset:  IM    
Wellman Laboratories of Photomedicine, Department of Dermatology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114, USA.
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MeSH Terms
Acute Disease
Administration, Oral
Apoptosis / radiation effects
Bromodeoxyuridine / metabolism
Cell Division
Dermatitis / etiology,  prevention & control*
Epidermis / drug effects,  pathology*,  radiation effects*
Keratinocytes / metabolism,  physiology
Lutein / administration & dosage*
Mice, Hairless
Proliferating Cell Nuclear Antigen / metabolism
Radiation Injuries / complications,  prevention & control*
Skin / metabolism
Ultraviolet Rays / adverse effects*
beta Carotene / administration & dosage*,  analogs & derivatives*
Reg. No./Substance:
0/Proliferating Cell Nuclear Antigen; 0/Xanthophylls; 127-40-2/Lutein; 144-68-3/zeaxanthin; 59-14-3/Bromodeoxyuridine; 7235-40-7/beta Carotene
Comment In:
J Invest Dermatol. 2003 Aug;121(2):viii   [PMID:  12880447 ]

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