Document Detail

Dietary conjugated linoleic acid increases PPAR gamma gene expression in adipose tissue of obese rat, and improves insulin resistance.
MedLine Citation:
PMID:  18304850     Owner:  NLM     Status:  MEDLINE    
Conjugated linoleic acid (CLA) is a class of positional, geometric, conjugated dienoic isomers of linoleic acid. Dietary CLA supplementation has resulted in a dramatic decrease in body fat mass in mice. However, some but not all studies in mice and humans have found that CLA promoted insulin resistance, and there were conflicting reports on the effects of CLA on peroxisomal proliferator-activated receptor-gamma (PPAR gamma) activation and expression. The objective of present study was to investigate the effect of CLA on insulin resistance and its molecular mechanisms. Fifty male Wistar rats were randomly designed to the control, high-fat and high-fat with CLA (0.75, 1.50, and 3.00 g in per 100 g diet) groups. The effect of CLA on insulin sensitivity and the mechanism of resisting diabetes by CLA were investigated by RT-PCR assay. The results showed that supplementation with CLA significantly reduced body weight gain and white fat pad weight in the rats, the levels of plasma free fatty acids (FFA), triglycerides (TGs), cholesterin (TC), leptin, insulin and blood glucose concentration in the obese rats of CLA group were also decreased compared to the rats in the high-fat group. Dietary CLA increased the mRNA expression of PPAR gamma, fatty acid binding proteins (aP2), fatty acid transporter protein (FATP), acyl-CoA synthetase (ACS) and adiponectin in the adipose tissues of obese rats. The results suggest that CLA may ameliorate insulin resistance by activating PPAR gamma, and increasing the expression of PPAR gamma target genes such as ap2, FATP, FAT, and adiponectin in the white adipose tissue.
Xiao-Rong Zhou; Chang-Hao Sun; Jia-Ren Liu; Dan Zhao
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-03-04
Journal Detail:
Title:  Growth hormone & IGF research : official journal of the Growth Hormone Research Society and the International IGF Research Society     Volume:  18     ISSN:  1096-6374     ISO Abbreviation:  Growth Horm. IGF Res.     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-07-14     Completed Date:  2008-10-31     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9814320     Medline TA:  Growth Horm IGF Res     Country:  Scotland    
Other Details:
Languages:  eng     Pagination:  361-8     Citation Subset:  IM    
Department of Nutrition and Food Hygiene, Public Health College of Harbin Medical University, NanGang District, Harbin, PR China.
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MeSH Terms
Adiponectin / genetics,  metabolism
Adipose Tissue / metabolism*
Fatty Acid-Binding Proteins / genetics,  metabolism
Gene Expression / drug effects*
Insulin Resistance / genetics*
Linoleic Acids, Conjugated / administration & dosage*,  pharmacology
Obesity / genetics,  metabolism
PPAR gamma / genetics*,  metabolism
RNA, Messenger / metabolism
Rats, Wistar
Reg. No./Substance:
0/Adiponectin; 0/Fatty Acid-Binding Proteins; 0/Linoleic Acids, Conjugated; 0/PPAR gamma; 0/RNA, Messenger; 0/Slc27a1 protein, rat

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