| Dietary carbohydrate to protein ratio affects TOR signaling and metabolism-related gene expression in the liver and muscle of rainbow trout after a single meal. | |
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MedLine Citation:
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PMID: 21209382 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Most teleost fish are known to require high levels of dietary proteins. Such high protein intake could have significant effects, particularly on insulin-regulated gene expression. We therefore analyzed the effects of an increase in the ratio of dietary carbohydrates/proteins on the refeeding activation of the Akt-TOR signaling pathways in rainbow trout and the effects on the expression of several genes related to hepatic and muscle metabolism and known to be regulated by insulin, amino acids and/or glucose. Fish were fed once one of three experimental diets containing high (H), medium (M) or low (L) protein (P) or carbohydrate (C) levels after 48h of feed deprivation. Activation of the Akt/TOR signaling pathway by refeeding was severely impaired by decreasing the proteins/carbohydrates ratio. Similarly, post-prandial regulation of several genes related to glucose (Glut4, glucose-6-phosphatase isoform 1), lipid (fatty acid synthase, ATP-citrate lyase, sterol responsive element binding protein, carnitine palmitoyltransferase 1 and 3-hydroxyacyl-CoA dehydrogenase) and amino acid metabolism (serine dehydratase and branched chain α-keto acid dehydrogenase E2 subunit) only occurred when fish were fed the high protein diet. On the other hand, diet composition had a low impact on the expression of genes related to muscle protein degradation. Interestingly, glucokinase was the only gene of those monitored whose expression was significantly up-regulated by increased carbohydrate intake. In conclusion, this study demonstrated that macro-nutrient composition of the diet strongly affected the insulin/amino acids signaling pathway and expression pattern of genes related to metabolism. |
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Authors:
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Iban Seiliez; Stephane Panserat; Marine Lansard; Sergio Polakof; Elisabeth Plagnes-Juan; Anne Surget; Karine Dias; Mélanie Larquier; Sadasivam Kaushik; Sandrine Skiba-Cassy |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-1-5 |
Journal Detail:
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Title: American journal of physiology. Regulatory, integrative and comparative physiology Volume: - ISSN: 1522-1490 ISO Abbreviation: - Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2011-1-6 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100901230 Medline TA: Am J Physiol Regul Integr Comp Physiol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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1INRA. |
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