| Dietary bile acid supplementation improves intestinal integrity and survival in a murine model. | |
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MedLine Citation:
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PMID: 20620329 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: In vitro supplementation of the bile salt, taurodeoxycholic acid (TDCA), has been shown to stimulate proliferation and prevent intestinal apoptosis in IEC-6 cells. We hypothesize that addition of TDCA to a rodent liquid diet will be protective against induced intestinal injury. METHODS: C57Bl6 mice were fed a liquid diet with or without 50-mg/(kg d) TDCA supplementation. After 6 days, the mice were injected with lipopolysaccharide (LPS) (10 mg/kg) to induce intestinal injury. Specimens were obtained 24 hours later and evaluated for intestinal apoptosis, crypt proliferation, and villus length. A separate cohort of animals was injected with LPS (25 mg/kg) and followed 7 days for survival. RESULTS: Mice whose diet was supplemented with TDCA had significantly increased survival. After LPS-induced injury, mice supplemented with TDCA showed decreased intestinal apoptosis by both H&E and caspase-3. They also had increased intestinal proliferation by 5-bromo-2'deoxyuridine staining and increased villus length. CONCLUSIONS: Dietary TDCA supplementation alleviates mucosal damage and improves survival after LPS-induced intestinal injury. Taurodeoxycholic acid is protective of the intestinal mucosa by increasing resistance to injury-induced apoptosis, stimulating enterocyte proliferation, and increasing villus length. Taurodeoxycholic acid supplementation also results in an increased survival benefit. Therefore, bile acid supplementation may potentially protect the intestine from injury or infection. |
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Authors:
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Erin E Perrone; Chen Chen; Shannon W Longshore; Oneybuchi Okezie; Brad W Warner; Chen-Chih Sun; Samuel M Alaish; Eric D Strauch |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of pediatric surgery Volume: 45 ISSN: 1531-5037 ISO Abbreviation: J. Pediatr. Surg. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-07-12 Completed Date: 2010-10-21 Revised Date: 2011-07-28 |
Medline Journal Info:
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Nlm Unique ID: 0052631 Medline TA: J Pediatr Surg Country: United States |
Other Details:
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Languages: eng Pagination: 1256-65 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Pediatric Surgery, University of Maryland Medical Center, Baltimore, MD 21201, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis / drug effects* Cell Proliferation / drug effects Cholagogues and Choleretics / administration & dosage*, therapeutic use Dietary Supplements* Disease Models, Animal Intestinal Mucosa / drug effects*, pathology Male Mice Mice, Inbred C57BL Short Bowel Syndrome / diet therapy*, mortality, pathology Survival Rate / trends Taurodeoxycholic Acid / administration & dosage*, therapeutic use Treatment Outcome |
| Grant Support | |
ID/Acronym/Agency:
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R21 DK075409-01A2/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Cholagogues and Choleretics; 516-50-7/Taurodeoxycholic Acid |
| Comments/Corrections | |
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