| Dietary Phosphatidylcholine Supplementation Attenuates Inflammatory Mucosal Damage in a Rat Model of Experimental Colitis. | |
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MedLine Citation:
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PMID: 22576006 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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ABSTRACT: This study was designed to follow the time course of inflammatory activation in a rodent model of 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced colitis. We hypothesized that oral phosphatidylcholine (PC) pretreatment regimens may influence leukocyte-mediated microcirculatory reactions in this condition.In series I Wistar rats were monitored 1 day after colitis induction (n=24), and in series II (n=24) on day 6 following a TNBS enema. The PC-pretreated animals received a 2% PC-enriched diet for 6 days before the TNBS enema (series I), or for 3 days before and 3 days after TNBS treatment (series II). The macrohemodynamics, serosal microcirculation (visualized by intravital videomicroscopy), colonic xanthine oxidoreductase, myeloperoxidase and nitric oxide (NO) end-products and changes in pro-inflammatory cytokine levels in plasma were measured. The mucosal structural injury was monitored in vivo by means of confocal laser scanning endomicroscopy.The TNBS enema induced a systemic hyperdynamic circulatory reaction with increased serosal capillary blood flow and significantly elevated colonic inflammatory enzyme activities, levels of NO production and cytokine concentrations. Acute colitis caused disruption of the capillary network whereas the morphological damage was less severe in series II. The PC pretreatment protocols led to significant decreases in the serosal hyperaemic reaction, the cytokine levels and the inflammatory enzyme activities. The objective signs of tissue damage were reduced in both series, and the number of mucus-producing goblet cells in the resolving phase of colitis was increased.Dietary PC efficiently decreases the cytokine-mediated progression of inflammatory events and preserves the microvascular structure in the large intestine. |
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Authors:
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Tamás Kovács; Gabriella Varga; Dániel Erces; Tünde Tőkés; László Tiszlavicz; Miklós Ghyczy; Mihály Boros; József Kaszaki |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-5-9 |
Journal Detail:
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Title: Shock (Augusta, Ga.) Volume: - ISSN: 1540-0514 ISO Abbreviation: - Publication Date: 2012 May |
Date Detail:
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Created Date: 2012-5-11 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9421564 Medline TA: Shock Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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1 Department of Paediatrics, Faculty of Medicine, University of Szeged, Hungary, 2 Institute Pathology, Faculty of Medicine, University of Szeged, Hungary; 4 retired chemist, Cologne, Germany. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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