Document Detail

Dietary phosphate restriction decreases stem cell proliferation and subsequent growth potential in neonatal pigs.
MedLine Citation:
PMID:  20053936     Owner:  NLM     Status:  MEDLINE    
Although mesenchymal stem cells (MSC) and satellite cells are essential for postnatal muscle and bone development and phosphate (PO(4)) restriction reduces both muscle and skeletal tissue growth, no research to our knowledge has investigated the possible mechanism by which this mineral may affect early cell programming. Twenty piglets obtained at 1 d of age (1.8 +/- 0.3 kg) received either a PO(4)-adequate diet or a 25% less PO(4)-available diet over a 15-d trial. Feed intake and body weight were recorded daily and blood samples collected every 5 d. After 15 d, pigs were given an intraperitoneal injection of bromodeoxyuridine 4 h prior to tissue collection. As expected, PO(4) deficiency resulted in reduced growth (P < 0.05), feed conversion efficiency (P < 0.05), and bone mineral content (P < 0.05), as well as lower plasma concentrations of both PO(4) (P < 0.01) and parathyroid hormone (P < 0.05). In addition to these classical indicators of PO(4) deficiency, there was also reduced proliferation of both MSC (P < 0.01) and satellite cells (P < 0.05) in vivo. The expression of osteocalcin mRNA in bone marrow was also 2-fold greater (P < 0.01) within the PO(4)-adequate treatment group. These data indicate that in addition to reductions in muscle and bone growth, dietary PO(4) affects proliferation of tissue-specific stem cells in vivo. Nutritional programming of tissue-specific stem cells by dietary PO(4) may have profound implications for life-long growth potential.
Lindsey S Alexander; Avanika Mahajan; Jack Odle; Kyle L Flann; Robert P Rhoads; Chad H Stahl
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Publication Detail:
Type:  Controlled Clinical Trial; Journal Article     Date:  2010-01-06
Journal Detail:
Title:  The Journal of nutrition     Volume:  140     ISSN:  1541-6100     ISO Abbreviation:  J. Nutr.     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-02-22     Completed Date:  2010-03-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0404243     Medline TA:  J Nutr     Country:  United States    
Other Details:
Languages:  eng     Pagination:  477-82     Citation Subset:  IM    
Laboratory of Developmental Nutrition, Department of Animal Science, North Carolina State University, Raleigh, NC 27695, USA.
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MeSH Terms
Animal Feed / analysis*
Animal Nutritional Physiological Phenomena
Bone Density / drug effects
Bone Marrow / drug effects,  metabolism
Cell Proliferation / drug effects*
Diet / veterinary*
Kidney / drug effects,  metabolism
Mesenchymal Stem Cells / drug effects*
Phosphorus / deficiency
Phosphorus, Dietary / pharmacology*
Swine / growth & development*
Weight Gain / drug effects
Reg. No./Substance:
0/Phosphorus, Dietary; 7723-14-0/Phosphorus

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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