Document Detail

Dietary n-6 polyunsaturated fatty acid deprivation increases docosahexaenoic acid metabolism in rat brain.
MedLine Citation:
PMID:  22117540     Owner:  NLM     Status:  MEDLINE    
Dietary n-6 polyunsaturated fatty acid (PUFA) deprivation in rodents reduces brain arachidonic acid (20:4n-6) concentration and 20:4n-6-preferring cytosolic phospholipase A(2) (cPLA(2) -IVA) and cyclooxygenase (COX)-2 expression, while increasing brain docosahexaenoic acid (DHA, 22:6n-3) concentration and DHA-selective calcium-independent phospholipase A(2) (iPLA(2) )-VIA expression. We hypothesized that these changes are accompanied by up-regulated brain DHA metabolic rates. Using a fatty acid model, brain DHA concentrations and kinetics were measured in unanesthetized male rats fed, for 15 weeks post-weaning, an n-6 PUFA 'adequate' (31.4 wt% linoleic acid) or 'deficient' (2.7 wt% linoleic acid) diet, each lacking 20:4n-6 and DHA. [1-(14) C]DHA was infused intravenously, arterial blood was sampled, and the brain was microwaved at 5 min and analyzed. Rats fed the n-6 PUFA deficient compared with adequate diet had significantly reduced n-6 PUFA concentrations in brain phospholipids but increased eicosapentaenoic acid (EPA, 20:5n-3), docosapentaenoic acid n-3 (DPAn-3, 22:5n-3), and DHA (by 9.4%) concentrations, particularly in ethanolamine glycerophospholipid (EtnGpl). Incorporation rates of unesterified DHA from plasma, which represent DHA metabolic loss from brain, were increased 45% in brain phospholipids, as was DHA turnover. Increased DHA metabolism following dietary n-6 PUFA deprivation may increase brain concentrations of antiinflammatory DHA metabolites, which with a reduced brain n-6 PUFA content, likely promotes neuroprotection and alters neurotransmission.
Miki Igarashi; Hyung-Wook Kim; Lisa Chang; Kaizong Ma; Stanley I Rapoport
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Intramural     Date:  2012-01-23
Journal Detail:
Title:  Journal of neurochemistry     Volume:  120     ISSN:  1471-4159     ISO Abbreviation:  J. Neurochem.     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-03-07     Completed Date:  2012-06-01     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  2985190R     Medline TA:  J Neurochem     Country:  England    
Other Details:
Languages:  eng     Pagination:  985-97     Citation Subset:  IM    
Copyright Information:
Published 2011. This article is a US Government work and is in the public domain in the USA.
Brain Physiology and Metabolism Section, National Institute on Aging, National Institutes of Health, Bethesda, Maryland, USA.
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MeSH Terms
Acyl Coenzyme A / metabolism
Animals, Newborn
Body Weight
Brain / metabolism*
Carbon Isotopes / pharmacokinetics
Chromatography, Gas
Chromatography, High Pressure Liquid / methods
Dietary Fats / administration & dosage*,  metabolism
Docosahexaenoic Acids / metabolism*,  pharmacokinetics
Fatty Acids, Omega-6 / deficiency*
Gene Expression Regulation / drug effects,  physiology*
Lipids / blood
Rats, Inbred F344
Grant Support
Reg. No./Substance:
0/Acyl Coenzyme A; 0/Carbon Isotopes; 0/Dietary Fats; 0/Fatty Acids, Omega-6; 0/Lipids; 25167-62-8/Docosahexaenoic Acids

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