| Dietary methionine restriction improves colon tight junction barrier function and alters claudin expression pattern. | |
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MedLine Citation:
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PMID: 20739626 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The beneficial effects of caloric restriction in increasing longevity and forestalling age-related diseases are well known. Dietary restriction of methionine also renders similar benefits. We recently showed in a renal epithelial cell culture system that reduction of culture medium methionine by 80% resulted in altered tight junctional (TJ) claudin composition and also improved epithelial barrier function (51). In the current study, we examined the effect of dietary restriction of methionine on TJ barrier function in rat gastrointestinal tissue to see whether this phenomenon also holds true in a tissue model and for a different epithelial cell type. After 28 days on methionine-restricted (MR) diet, rats showed small but significant reductions in the plasma and (intracellular) colonocyte levels of methionine. Colon mucosal sheets from rats on the MR diet showed increased transepithelial electrical resistance with concomitant decrease in paracellular diffusion of (14)C-D-mannitol, suggesting improved barrier function relative to rats on control diet. This improved barrier function could not be explained by changes in colon crypt length or frequency. Neither was the colonocyte mitotic index nor the apoptotic frequency altered significantly. However, TJ composition/structure was being altered by the MR diet. RT-PCR and Western blot analysis showed an increase in the abundance of claudin-3 and an apparent change in the posttranslational modification of occludin, data reinforcing a paracellular barrier alteration. Overall, our data suggest that reduction in dietary intake of methionine results in improved epithelial barrier function by inducing altered TJ protein composition. |
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Authors:
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Arivudainambi Ramalingam; Xuexuan Wang; Melissa Gabello; M Carmen Valenzano; Alejandro P Soler; Akihiro Ko; Patrice J Morin; James M Mullin |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-08-25 |
Journal Detail:
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Title: American journal of physiology. Cell physiology Volume: 299 ISSN: 1522-1563 ISO Abbreviation: Am. J. Physiol., Cell Physiol. Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-10-28 Completed Date: 2010-11-22 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100901225 Medline TA: Am J Physiol Cell Physiol Country: United States |
Other Details:
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Languages: eng Pagination: C1028-35 Citation Subset: IM |
Affiliation:
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Lankenau Institute for Medical Research, Wynnewood, PA 19096, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Body Weight Claudins / genetics, metabolism* Colon* / anatomy & histology, metabolism Diet* Intestinal Mucosa* / cytology, metabolism Male Membrane Proteins / genetics, metabolism Methionine / metabolism* Rats Rats, Sprague-Dawley Tight Junctions / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Claudins; 0/Membrane Proteins; 0/occludin; 63-68-3/Methionine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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