Document Detail


Dietary G-rutin suppresses glycation in tissue proteins of streptozotocin-induced diabetic rats.
MedLine Citation:
PMID:  14577587     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The present study focused on examining the efficacy of feeding a rutin-glucose derivative (G-rutin) to inhibit glycation reactions that can occur in muscle, kidney and plasma proteins of diabetic rats. Both thiobarbituric acid-reactive substance levels and protein carbonyl contents in muscle and kidney were significantly (p < 0.05) reduced in streptozotocin-induced diabetic rats fed G-rutin supplemented diet, compared to diabetic rats fed control diet. The N(epsilon)-fructoselysine content in muscle and kidney, a biomarker of early glycation reaction, was markedly (p < 0.05) increased by diabetes, but significantly (p < 0.05) reduced in diabetic rats fed G-rutin. Advanced glycation end-products (AGEs) in serum and kidney protein were measured by immunoblot using anti-AGE antibody, and were also reduced in diabetic rats fed dietary G-rutin. Feeding G-rutin also slightly inhibited aldose reductase activity in these animals. These results demonstrate for the first time that dietary G-rutin consumption can provide potential health benefits that are related to the inhibition of tissue glycation reactions common to diabetes.
Authors:
Takashi Nagasawa; Nobuaki Tabata; Yoshiaki Ito; Youichi Aiba; Naoyuki Nishizawa; David D Kitts
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Molecular and cellular biochemistry     Volume:  252     ISSN:  0300-8177     ISO Abbreviation:  Mol. Cell. Biochem.     Publication Date:  2003 Oct 
Date Detail:
Created Date:  2003-10-27     Completed Date:  2004-06-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0364456     Medline TA:  Mol Cell Biochem     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  141-7     Citation Subset:  IM    
Affiliation:
Food and Health Science, Faculty of Agriculture, Iwate University, Morioka, Iwate, Tokyo, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blotting, Western
Diabetes Mellitus, Experimental / metabolism*
Diet*
Glucose / metabolism*
Glycosylation End Products, Advanced
Lysine / analogs & derivatives*,  blood,  metabolism
Male
Rats
Rats, Wistar
Rutin / administration & dosage,  pharmacology*
Streptozocin
Chemical
Reg. No./Substance:
0/Glycosylation End Products, Advanced; 153-18-4/Rutin; 18883-66-4/Streptozocin; 21291-40-7/fructosyl-lysine; 50-99-7/Glucose; 56-87-1/Lysine

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