Document Detail


Diet supplemented with yoghurt or milk fermented by Lactobacillus casei DN-114 001 stimulates growth and brush-border enzyme activities in mouse small intestine.
MedLine Citation:
PMID:  9693207     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The nutritional benefits of lactic acid bacteria in fermented dairy products have been well documented, especially in terms of weight gain and feed efficiency, but not in terms of small intestine adaptation. The effects of a diet supplemented (30% wt/wt) with milk fermented either by Lactobacillus casei DN-114 001 or yoghurt for 3 or 15 days were investigated in the small intestine of mice by morphometry, kinetic analysis and determination of brush-border enzyme activities. Results were compared with those obtained with standard or milk isocaloric diets. Cell proliferation and villous area were significantly increased in the proximal intestine of mice fed the fermented-milk-supplemented diets for 3 days and were associated with hypertrophy and hyperplasia of Paneth and goblet cells. Lactase-specific activity was increased by fermented-milk diets at days 3 and 15, whereas there was no variation in maltase-specific activity. Alkaline phosphatase-specific activity was increased after 3 days of the three tested diets in the whole intestine, and after 15 days in the proximal intestine. Aminopeptidase activity was increased in the distal part of the intestine after 3 days of the 3 diets. Our findings suggest that diets supplemented with fermented milks have a positive effect on the trophicity of the mucosa in the small intestine of mice.
Authors:
K Thoreux; D Balas; C Bouley; F Senegas-Balas
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Digestion     Volume:  59     ISSN:  0012-2823     ISO Abbreviation:  Digestion     Publication Date:    1998 Jul-Aug
Date Detail:
Created Date:  1998-09-09     Completed Date:  1998-09-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0150472     Medline TA:  Digestion     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  349-59     Citation Subset:  IM    
Affiliation:
Laboratoire d'Histologie, Faculté de Médecine, Nice, France. thoreux@unice.fr
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MeSH Terms
Descriptor/Qualifier:
Alkaline Phosphatase / metabolism
Animals
Body Weight
Cell Division
Dietary Supplements*
Hydrolases / metabolism*
Intestinal Mucosa / cytology,  enzymology
Intestine, Small / cytology*,  enzymology
Lactase
Lactobacillus casei
Mice
Microvilli / enzymology*
Milk*
Weight Gain
Yogurt*
beta-Galactosidase / metabolism
Chemical
Reg. No./Substance:
EC 3.-/Hydrolases; EC 3.1.3.1/Alkaline Phosphatase; EC 3.2.1.108/Lactase; EC 3.2.1.23/beta-Galactosidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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