| Diet-specific salivary gene expression and glucose oxidase activity in Spodoptera exigua (Lepidoptera: Noctuidae) larvae. | |
| | |
MedLine Citation:
|
PMID: 20688075 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
|
Saliva secreted during caterpillar feeding contains enzymes to initiate digestion or detoxify noxious plant compounds. Activity of some salivary enzymes is diet-dependent and may be transcriptionally regulated. In this study, cDNA-amplified fragment length polymorphism was used to identify beet armyworm, Spodoptera exigua Hübner, labial salivary genes that are differentially expressed in response to diet. In addition, SeGOX was sequenced based on homology and characterized to confirm that the transcript encodes a functional enzyme. Three labial salivary transcripts, encoding glucose oxidase (GOX) and two proteins of unknown function (Se1H and Se2J), were expressed in a diet-specific manner. Since diet, particularly the protein to digestible carbohydrate levels and ratio, may affect labial salivary enzyme activity, the influence of nutritional quality on gene expression was determined. Transcript levels of the labial salivary genes Se1H, Se2J and SeGOX increased with dietary carbohydrate levels, regardless of protein concentrations. In contrast GOX enzymatic activity increased with increasing dietary carbohydrates when caterpillars were fed protein-rich diets, but not when caterpillars were fed protein-poor diets. Our results suggest that dietary carbohydrates affect SeGOX, Se1H and Se2J transcription, but dietary protein or amino acid levels affect translational and/or post-translational regulation of the enzyme GOX. |
| | |
Authors:
|
Khashayar Afshar; Philippe J Dufresne; Li Pan; Magali Merkx-Jacques; Jacqueline C Bede |
Related Documents
:
|
3841735 - Paraventricular nucleus injections of peptide yy and neuropeptide y preferentially enha... 19821225 - Influence of protein- versus carbohydrate-enriched feedings on physiological responses ... 22421895 - Air-displacement plethysmography pediatric option in 2-6 years old using the four-compa... 11281505 - Effect of long-term intake of asian food with different glycemic indices on diabetic co... 10882815 - Sucralose: lack of effects on sperm glycolysis and reproduction in the rat. 3867905 - Serum lipids and lipoprotein abnormalities in patients with thrombotic stroke--with exp... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-08-08 |
Journal Detail:
|
Title: Journal of insect physiology Volume: 56 ISSN: 1879-1611 ISO Abbreviation: J. Insect Physiol. Publication Date: 2010 Dec |
Date Detail:
|
Created Date: 2010-10-29 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 2985080R Medline TA: J Insect Physiol Country: England |
Other Details:
|
Languages: eng Pagination: 1798-806 Citation Subset: IM |
Copyright Information:
|
Copyright © 2010 Elsevier Ltd. All rights reserved. |
Affiliation:
|
Department of Plant Science, McGill University, 21,111 Lakeshore, Ste-Anne-de-Bellevue, Québec, Canada. Khashayar.afshar@mail.mcgill.ca |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Surgically increased ovarian mass in the honey bee confirms link between reproductive physiology and...
Next Document: Total body nitrogen and total body carbon as indicators of body protein and body lipids in the melon...