| Diet influences the colonisation of Campylobacter jejuni and distribution of mucin carbohydrates in the chick intestinal tract. | |
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MedLine Citation:
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PMID: 11130183 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The objectives of this study were to evaluate the effect of diet on the colonisation by Campylobacter jejuni of the chick caeca, and to determine whether the viscosity of the intestinal contents and mucin carbohydrates were altered by the diet. The diets investigated were maize based, wheat-based or wheat-based supplemented with xylanase. The xylanase-supplemented diet reduced the viscosity and lowered the numbers of Camp. jejuni. Feeding the enzyme-supplemented diet increased the amount of neutral and sulphated mucins in the goblet cells of the small and large intestines and caecum. An abundance of sulphated and carboxylated mucins was seen in the surface goblet cells of the large intestine with the maize- and wheat-based diets. Both the diet supplemented with xylanase and the maize diets increased crypt-surface glycosylation of the sialic acid residues. The analysed data from the combined sites showed significant differences in the amount of neutral and acidic mucins when comparing the wheat and the wheat plus xylanase diets. However, no changes were shown in the staining intensity of sulphated mucins between the three diets. Significant differences in the glycosylation of sialic acid and in the N-acetylglucosamine residues were shown between dietary groups. These results provide evidence that the wheat diet supplemented with xylanase leads to greater changes in the mucin composition and carbohydrate expression of goblet cell glycoconjugates, which are associated with a reduction in intestinal viscosity and decreased numbers of Camp. jejuni. |
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Authors:
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F Fernandez; R Sharma; M Hinton; M R Bedford |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Cellular and molecular life sciences : CMLS Volume: 57 ISSN: 1420-682X ISO Abbreviation: Cell. Mol. Life Sci. Publication Date: 2000 Nov |
Date Detail:
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Created Date: 2000-12-20 Completed Date: 2001-01-04 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9705402 Medline TA: Cell Mol Life Sci Country: Switzerland |
Other Details:
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Languages: eng Pagination: 1793-801 Citation Subset: IM |
Affiliation:
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Department of Clinical Veterinary Science, University of Bristol, North Somerset, UK. fresie.fernandez@bristol.ac.uk |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Campylobacter jejuni / growth & development* Carbohydrate Sequence Chickens Colony Count, Microbial Diet* Histocytochemistry Intestines / metabolism*, microbiology* Lectins / metabolism Molecular Sequence Data Mucins / chemistry, metabolism* Viscosity |
| Chemical | |
Reg. No./Substance:
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0/Lectins; 0/Mucins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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