Document Detail

Diet-induced adiposity alters the serum profile of inflammation in C57BL/6N mice as measured by antibody array.
MedLine Citation:
PMID:  19267713     Owner:  NLM     Status:  MEDLINE    
Morbid obesity is considered a systemic inflammatory state. The objective of this project was to characterize the adipokine, cytokine and chemokine protein profile in serum from control, lean and obese mice. We hypothesized that chemokines and cytokines are altered by caloric restriction and diet-induced obesity as a function of changes in body composition. Six-week-old female C57BL/6N mice (n = 12 per group) were randomized to one of three diets: control (fed ad libitum); lean (30% calorie-restricted regimen relative to control) and diet-induced obese (DIO; high calorie diet, fed ad libitum). Body weight, body composition and food intake were monitored throughout the study. After 10 weeks on the diets, blood samples were collected, and adipokine/cytokine/chemokine serum profiles were measured by antibody array. Lean mice, relative to the control group, displayed increased concentrations of insulin-like growth factor (IGF) binding protein-3, -5 and -6 and adiponectin and decreased IGF-1. These mice also showed increased concentrations of interleukin (IL)-10, IL-12 p40/p70, eotaxin, monocyte chemoattractant protein-5 and SDF-1. In contrast, DIO mice displayed increased leptin, IL-6 and LPS-induced chemokine and decreased concentrations of all chemokines/cytokines measured relative to control mice. As such, these data indicate that DIO may lead to an inflammatory state characterized as a shift towards a T helper lymphocyte type 1-skewed responsiveness. The demonstration of differential adipokine, cytokine and chemokine protein profile in control, lean and DIO mice may have implications for immune responsiveness and risk of disease.
J I Fenton; N P Nu??ez; S Yakar; S N Perkins; N G Hord; S D Hursting
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Diabetes, obesity & metabolism     Volume:  11     ISSN:  1463-1326     ISO Abbreviation:  Diabetes Obes Metab     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-03-09     Completed Date:  2010-01-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100883645     Medline TA:  Diabetes Obes Metab     Country:  England    
Other Details:
Languages:  eng     Pagination:  343-54     Citation Subset:  IM    
Department of Food Science and Human Nutrition, Michigan State University, East Lansing, MI 48824, USA.
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MeSH Terms
Adipokines / blood
Adiposity / physiology
Body Composition / physiology
Body Weight / physiology
Chemokines / blood
Cytokines / blood*
Diet / adverse effects*
Disease Models, Animal
Energy Metabolism / physiology
Inflammation Mediators / blood*
Mice, Inbred C57BL
Obesity / blood*,  etiology
Protein Array Analysis / methods
Random Allocation
Reagent Kits, Diagnostic
Reproducibility of Results
Reg. No./Substance:
0/Adipokines; 0/Chemokines; 0/Cytokines; 0/Inflammation Mediators; 0/Reagent Kits, Diagnostic

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