Document Detail

Diet- and colonization-dependent intestinal dysfunction predisposes to necrotizing enterocolitis in preterm pigs.
MedLine Citation:
PMID:  16697741     Owner:  NLM     Status:  MEDLINE    
BACKGROUND & AIMS: Preterm birth and formula feeding are key risk factors associated with necrotizing enterocolitis (NEC) in infants, but little is known about intestinal conditions that predispose to disease. Thus, structural, functional, and microbiologic indices were used to investigate the etiology of spontaneous NEC development in preterm pigs. METHODS: Piglets were delivered by cesarean section at 92% gestation, reared in infant incubators, and fed infant formula or colostrum every 3 hours (n = 120) until tissue collection at 1-2 days of age. RESULTS: Clinical and histopathologic signs of NEC were observed in 57% of pigs fed FORMULA (26/46) and in 5% of pigs fed COLOSTRUM (2/38) (P < .05). Relative to COLOSTRUM, both healthy and sick FORMULA pigs had reduced intestinal villous heights, enzyme activities, nutrient absorption, and antioxidant levels and higher inducible nitric oxide synthetase activity (P < .05). In healthy pigs, mucosal microbial diversity remained low and diet independent. NEC pigs showed bacterial overgrowth, and a high mucosal density of Clostridium perfringens was detected in some but not all pigs. Germ-free conditions and antiserum against Clostridium perfringens toxin prevented intestinal dysfunction and NEC in formula-fed pigs, whereas the gut trophic factors, epidermal growth factor, and glucagon-like peptide 2 had limited effects. CONCLUSIONS: A subclinical, formula-induced mucosal atrophy and dysfunction predispose to NEC and bacterial overgrowth. The adverse feeding effects are colonization dependent and may be reduced by factors in colostrum that include antibodies against aggressive toxins such as those of Clostridium perfringens.
Per T Sangild; Richard H Siggers; Mette Schmidt; Jan Elnif; Charlotte R Bjornvad; Thomas Thymann; Marie L Grondahl; Axel K Hansen; Soeren K Jensen; Mette Boye; Lars Moelbak; Randal K Buddington; Björn R Weström; Jens J Holst; Douglas G Burrin
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Gastroenterology     Volume:  130     ISSN:  0016-5085     ISO Abbreviation:  Gastroenterology     Publication Date:  2006 May 
Date Detail:
Created Date:  2006-05-15     Completed Date:  2006-06-22     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0374630     Medline TA:  Gastroenterology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1776-92     Citation Subset:  AIM; IM    
Divisions of Nutrition and Reproduction, Royal Veterinary and Agricultural University, Frederiksberg, Denmark.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Animals, Newborn
Base Sequence
Biopsy, Needle
Colony Count, Microbial
DNA, Bacterial / analysis
Enterocolitis, Necrotizing / epidemiology*,  pathology,  prevention & control*
Gastrointestinal Tract / microbiology
Infant Formula / administration & dosage*
Intestinal Absorption / physiology
Intestinal Mucosa / microbiology,  pathology
Intestine, Small / embryology,  metabolism*,  pathology
Molecular Sequence Data
Polymerase Chain Reaction / methods
Pregnancy, Animal*
Premature Birth
Risk Factors
Sensitivity and Specificity
Grant Support
Reg. No./Substance:
0/DNA, Bacterial

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Candida albicans is an immunogen for anti-Saccharomyces cerevisiae antibody markers of Crohn's disea...
Next Document:  Drug and bile acid transporters in rosuvastatin hepatic uptake: function, expression, and pharmacoge...