| Diesel exhaust particles are mutagenic in FE1-MutaMouse lung epithelial cells. | |
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MedLine Citation:
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PMID: 18423769 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The particulate phase of diesel engine exhaust is likely carcinogenic. However, the mechanisms of diesel exhaust particles (DEPs) induced mutagenicity/carcinogenicity are still largely unknown. We determined the mutant frequency following eight repeated 72 h incubations with 37.5 or 75 microg/ml DEP (NIST SRM 1650) in the FE1-MutaMouse lung epithelial cell line. We measured DEP-induced acellular and intracellular production of reactive oxygen species (ROS) and compared with ROS production induced by carbon black, which we have previously shown is mutagenic in this cell line [N.R. Jacobsen, A.T. Saber, P. White, P. Moller, G. Pojana, U. Vogel, S. Loft, J. Gingerich, L. Soper, G.R. Douglas, H. Wallin. Increased mutant frequency by carbon black, but not quartz, in the lacZ and cII transgenes of mutamouse lung epithelial cells, Environ. Mol. Mutagen. 48(6) (2007) 451-461]. The mutant frequency was marginally elevated in cells treated with 37.5 microg/ml DEP (1.29-fold [95% CI: 0.96-1.60], p=0.08) and significantly increased in cells treated with 75 microg/ml DEP (1.55-fold [95% CI: 1.23-1.87], p < 0.001). ROS production from DEP was low both within cells and in acellular systems when compared to carbon black. These results show that DEP are mutagenic in a mammalian cell line in vitro and that additional pathways besides ROS production, such as those involving the presence of polycyclic aromatic hydrocarbons, likely are involved in the mutagenesis. |
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Authors:
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Nicklas Raun Jacobsen; Peter Møller; Corey Alexander Cohn; Steffen Loft; Ulla Vogel; Håkan Wallin |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-03-18 |
Journal Detail:
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Title: Mutation research Volume: 641 ISSN: 0027-5107 ISO Abbreviation: Mutat. Res. Publication Date: 2008 May |
Date Detail:
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Created Date: 2008-04-29 Completed Date: 2008-07-08 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0400763 Medline TA: Mutat Res Country: Netherlands |
Other Details:
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Languages: eng Pagination: 54-7 Citation Subset: IM |
Affiliation:
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National Research Centre for the Working Environment, Lersø Parkallé 105, DK-2100 Copenhagen Ø, Denmark. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Air Pollutants
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toxicity* Animals Cells, Cultured Epithelial Cells / drug effects*, metabolism Lung / drug effects*, metabolism Mice Mutagenesis Mutagens / toxicity* Reactive Oxygen Species / metabolism Soot / toxicity* Vehicle Emissions / toxicity* |
| Chemical | |
Reg. No./Substance:
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0/Air Pollutants; 0/Mutagens; 0/Reactive Oxygen Species; 0/Soot; 0/Vehicle Emissions |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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