Document Detail


Diesel exhaust particles are mutagenic in FE1-MutaMouse lung epithelial cells.
MedLine Citation:
PMID:  18423769     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The particulate phase of diesel engine exhaust is likely carcinogenic. However, the mechanisms of diesel exhaust particles (DEPs) induced mutagenicity/carcinogenicity are still largely unknown. We determined the mutant frequency following eight repeated 72 h incubations with 37.5 or 75 microg/ml DEP (NIST SRM 1650) in the FE1-MutaMouse lung epithelial cell line. We measured DEP-induced acellular and intracellular production of reactive oxygen species (ROS) and compared with ROS production induced by carbon black, which we have previously shown is mutagenic in this cell line [N.R. Jacobsen, A.T. Saber, P. White, P. Moller, G. Pojana, U. Vogel, S. Loft, J. Gingerich, L. Soper, G.R. Douglas, H. Wallin. Increased mutant frequency by carbon black, but not quartz, in the lacZ and cII transgenes of mutamouse lung epithelial cells, Environ. Mol. Mutagen. 48(6) (2007) 451-461]. The mutant frequency was marginally elevated in cells treated with 37.5 microg/ml DEP (1.29-fold [95% CI: 0.96-1.60], p=0.08) and significantly increased in cells treated with 75 microg/ml DEP (1.55-fold [95% CI: 1.23-1.87], p < 0.001). ROS production from DEP was low both within cells and in acellular systems when compared to carbon black. These results show that DEP are mutagenic in a mammalian cell line in vitro and that additional pathways besides ROS production, such as those involving the presence of polycyclic aromatic hydrocarbons, likely are involved in the mutagenesis.
Authors:
Nicklas Raun Jacobsen; Peter Møller; Corey Alexander Cohn; Steffen Loft; Ulla Vogel; Håkan Wallin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-03-18
Journal Detail:
Title:  Mutation research     Volume:  641     ISSN:  0027-5107     ISO Abbreviation:  Mutat. Res.     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-04-29     Completed Date:  2008-07-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0400763     Medline TA:  Mutat Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  54-7     Citation Subset:  IM    
Affiliation:
National Research Centre for the Working Environment, Lersø Parkallé 105, DK-2100 Copenhagen Ø, Denmark.
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MeSH Terms
Descriptor/Qualifier:
Air Pollutants / toxicity*
Animals
Cells, Cultured
Epithelial Cells / drug effects*,  metabolism
Lung / drug effects*,  metabolism
Mice
Mutagenesis
Mutagens / toxicity*
Reactive Oxygen Species / metabolism
Soot / toxicity*
Vehicle Emissions / toxicity*
Chemical
Reg. No./Substance:
0/Air Pollutants; 0/Mutagens; 0/Reactive Oxygen Species; 0/Soot; 0/Vehicle Emissions

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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