Document Detail

Diesel exhaust particles are mutagenic in FE1-MutaMouse lung epithelial cells.
MedLine Citation:
PMID:  18423769     Owner:  NLM     Status:  MEDLINE    
The particulate phase of diesel engine exhaust is likely carcinogenic. However, the mechanisms of diesel exhaust particles (DEPs) induced mutagenicity/carcinogenicity are still largely unknown. We determined the mutant frequency following eight repeated 72 h incubations with 37.5 or 75 microg/ml DEP (NIST SRM 1650) in the FE1-MutaMouse lung epithelial cell line. We measured DEP-induced acellular and intracellular production of reactive oxygen species (ROS) and compared with ROS production induced by carbon black, which we have previously shown is mutagenic in this cell line [N.R. Jacobsen, A.T. Saber, P. White, P. Moller, G. Pojana, U. Vogel, S. Loft, J. Gingerich, L. Soper, G.R. Douglas, H. Wallin. Increased mutant frequency by carbon black, but not quartz, in the lacZ and cII transgenes of mutamouse lung epithelial cells, Environ. Mol. Mutagen. 48(6) (2007) 451-461]. The mutant frequency was marginally elevated in cells treated with 37.5 microg/ml DEP (1.29-fold [95% CI: 0.96-1.60], p=0.08) and significantly increased in cells treated with 75 microg/ml DEP (1.55-fold [95% CI: 1.23-1.87], p < 0.001). ROS production from DEP was low both within cells and in acellular systems when compared to carbon black. These results show that DEP are mutagenic in a mammalian cell line in vitro and that additional pathways besides ROS production, such as those involving the presence of polycyclic aromatic hydrocarbons, likely are involved in the mutagenesis.
Nicklas Raun Jacobsen; Peter Møller; Corey Alexander Cohn; Steffen Loft; Ulla Vogel; Håkan Wallin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-03-18
Journal Detail:
Title:  Mutation research     Volume:  641     ISSN:  0027-5107     ISO Abbreviation:  Mutat. Res.     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-04-29     Completed Date:  2008-07-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0400763     Medline TA:  Mutat Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  54-7     Citation Subset:  IM    
National Research Centre for the Working Environment, Lersø Parkallé 105, DK-2100 Copenhagen Ø, Denmark.
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MeSH Terms
Air Pollutants / toxicity*
Cells, Cultured
Epithelial Cells / drug effects*,  metabolism
Lung / drug effects*,  metabolism
Mutagens / toxicity*
Reactive Oxygen Species / metabolism
Soot / toxicity*
Vehicle Emissions / toxicity*
Reg. No./Substance:
0/Air Pollutants; 0/Mutagens; 0/Reactive Oxygen Species; 0/Soot; 0/Vehicle Emissions

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