Document Detail

Dielectrophoretic segregation of different human cell types on microscope slides.
MedLine Citation:
PMID:  15859584     Owner:  NLM     Status:  MEDLINE    
A new method for preparing cells for microscopic examination is presented in which cell mixtures are fractionated by dielectrophoretic forces and simultaneously collected into characteristic zones on slides. The method traps cells directly from the suspending medium onto the slide, reducing cell loss. Furthermore, it exploits differences in the dielectric properties of the cells, which sensitively reflect their morphology. Because different cell types are trapped in characteristic zones on the slide, the technique represents an advance over existing methods for slide preparation, such as centrifugation and smears where cells are randomly distributed. In particular, the new method should aid in the detection of rare and anomalous cell subpopulations that might otherwise go unnoticed against a high background of normal cells. As well as being suitable for traditional microscopic examination and automated slide scanning approaches, it is compatible with histochemical and immunochemical techniques, as well as emerging molecular and proteomic methods. This paper describes the rationale and design of this so-called electrosmear instrumentation and shows experimental results that verify the theory and applicability of the method with model cell lines and normal peripheral blood subpopulations.
Chandra M Das; Frederick Becker; Suzanne Vernon; Jamileh Noshari; Celine Joyce; Peter R C Gascoyne
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Analytical chemistry     Volume:  77     ISSN:  0003-2700     ISO Abbreviation:  Anal. Chem.     Publication Date:  2005 May 
Date Detail:
Created Date:  2005-04-29     Completed Date:  2007-02-06     Revised Date:  2013-06-09    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2708-19     Citation Subset:  IM    
Department of Molecular Pathology, University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, Texas 77030, USA.
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MeSH Terms
Cell Separation / instrumentation,  methods*
Electrophoresis / instrumentation,  methods*
HL-60 Cells
Jurkat Cells
Grant Support
IPA09663 01//PHS HHS; R01-DK51065/DK/NIDDK NIH HHS; R33 CA088364-04/CA/NCI NIH HHS

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