Document Detail

Dielectrophoretic oocyte selection chip for in vitro fertilization.
MedLine Citation:
PMID:  18071907     Owner:  NLM     Status:  MEDLINE    
This paper reports a new dielectrophoretic separation method of porcine oocytes for in vitro fertilization. Conventional manual selection of oocyte highly depends on the expert's experience and lacks universal standards for identifying the quality of oocyte. In this study, an electrode array chip with castellated shape was developed to evaluate dielectrophoretic velocities of oocytes, under applied bias conditions with an AC 3 V waveform at 1 MHz for 15 s. Based on different dielectrophoresis (DEP) response, the selected group of oocytes that moved showed a better developmental potential than the group of oocytes that stayed, representing a higher rate of blastocyst formation and a lower rate of polyspermic fertilization. In addition, the overall developmental potential of oocytes selected by the DEP device was comparable to that of oocytes selected by conventional manual method. These results demonstrate that the difference in dielectrophoretic velocity can be used to establish an objective criterion for the selection of oocytes. Consequently, this method will open the possibility to develop an automatic tool for oocyte selection, which would be helpful for assisted reproductive technologies such as transgenic and clonal animal production.
Wonjae Choi; Ji-Su Kim; Do-Hyun Lee; Kyung-Kwang Lee; Deog-Bon Koo; Je-Kyun Park
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biomedical microdevices     Volume:  10     ISSN:  1387-2176     ISO Abbreviation:  Biomed Microdevices     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-04-14     Completed Date:  2008-07-31     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100887374     Medline TA:  Biomed Microdevices     Country:  United States    
Other Details:
Languages:  eng     Pagination:  337-45     Citation Subset:  IM    
Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), 335 Gwahangno, Yuseong-gu, Daejeon, 305-701, Republic of Korea.
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MeSH Terms
Cell Culture Techniques / instrumentation*,  methods
Cell Separation / instrumentation*,  methods
Cells, Cultured
Electrophoresis / instrumentation*,  methods
Equipment Design
Equipment Failure Analysis
Fertilization in Vitro / instrumentation*,  methods
Microfluidic Analytical Techniques / instrumentation*,  methods
Oocytes / cytology*,  physiology*

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