| Dielectrophoretic concentration of low-abundance nanoparticles using a nanostructured tip. | |
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MedLine Citation:
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PMID: 23137928 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Electric field-induced concentration has the potential for application in highly sensitive detection of nanoparticles (NPs) for disease diagnosis and drug discovery. Conventional two-dimensional planar electrodes, however, have shown limited sensitivity in NP concentration. In this paper, the dielectrophoretic (DEP) concentration of low-abundance NPs is studied using a nanostructured tip where a high electric field of 3 × 10(7) V m(-1) is generated. In experimental studies, individual 2, 10, and 100 nm Au NPs are concentrated to a nanotip using DEP concentration and are detected by scanning transmission and scanning electron microscopes. The DEP force on Au NPs near the end of a nanotip is computed according to the distance, and then compared with Brownian motion-induced force. The computational study shows qualitative agreement with the experimental results. When the experimental conditions for DEP concentration are optimized for 8 nm-long oligonucleotides, the sensitivity of a nanotip is 10 aM (10 attomolar; nine copies in a 1.5 μl sample volume). This DEP concentrator using a nanotip can be used for molecular detection without amplification. |
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Authors:
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Woon-Hong Yeo; Adrian M Kopacz; Jong-Hoon Kim; Xinqi Chen; Jinsong Wu; Dayong Gao; Kyong-Hoon Lee; Wing-Kam Liu; Jae-Hyun Chung |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-11-09 |
Journal Detail:
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Title: Nanotechnology Volume: 23 ISSN: 1361-6528 ISO Abbreviation: Nanotechnology Publication Date: 2012 Nov |
Date Detail:
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Created Date: 2012-11-9 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101241272 Medline TA: Nanotechnology Country: - |
Other Details:
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Languages: ENG Pagination: 485707 Citation Subset: - |
Affiliation:
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Department of Mechanical Engineering, University of Washington, Seattle, WA 98195, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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