Document Detail


Dielectrophoretic characterisation of Friend murine erythroleukaemic cells as a measure of induced differentiation.
MedLine Citation:
PMID:  2328267     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Dielectrophoresis measurements, the study of the motion of particles in non-uniform a.c. electrical fields, have been made on three cell lines (DS19, R1 and DR1) of Friend murine erythroleukaemia cells as a function of hexamethylene bisacetamide (HMBA) treatment. The effects of saponin treatment on R1 cells and neuraminidase on human red blood cells were also studied. It is shown that the dielectrophoretic behaviour can be interpreted in terms of cell surface charge and cell membrane conductivity effects. HMBA reduces the cell surface charge on all three cell lines, and in lines DS19 and DR10, where the cells are induced to differentiate, there is an increase in effective cell conductivity. This gain in conductivity is concluded to be associated with either an enhanced lateral electrophoretic motion of delocalised ions or of the polarisability of dipoles at the membrane surface.
Authors:
J P Burt; R Pethig; P R Gascoyne; F F Becker
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1034     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1990 Apr 
Date Detail:
Created Date:  1990-05-30     Completed Date:  1990-05-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  93-101     Citation Subset:  IM    
Affiliation:
Institute of Molecular and Biomolecular Electronics, University of Wales, Bangor, Gwynedd, U.K.
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MeSH Terms
Descriptor/Qualifier:
Acetamides / pharmacology
Animals
Cell Differentiation / drug effects
Cell Line
Cell Membrane / physiology*
Electric Conductivity
Electrophoresis
Friend murine leukemia virus
Leukemia, Erythroblastic, Acute / pathology*
Mice
Chemical
Reg. No./Substance:
0/Acetamides; 3073-59-4/hexamethylene bisacetamide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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