Document Detail


Dielectrophoretic capture of mammalian cells using transparent indium tin oxide electrodes in microfluidic systems.
MedLine Citation:
PMID:  19130589     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Transparent indium tin oxide microelectrodes were fabricated and used to immobilize suspended NIH 3T3 fibroblast cells by positive dielectrophoresis. The indium tin oxide electrodes facilitated microscopic observation of immobilized cells compared with opaque metallized electrodes. Dielectrophoresis was used to capture arrays of individual cells and form small cell clusters within a microfluidic network. The extent of cellular immobilization (no-cell, single-cell, or multiple-cell capture) was correlated with the applied voltage and inversely with the flow velocity. Specific conditions yielding predominantly single-cell capture were identified. The viability of immobilized cells was confirmed using fluorescence microscopy.
Authors:
Bharat Sankaran; Milena Racic; Alessandro Tona; Mulpuri V Rao; Michael Gaitan; Samuel P Forry
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Electrophoresis     Volume:  29     ISSN:  1522-2683     ISO Abbreviation:  Electrophoresis     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2009-01-14     Completed Date:  2009-02-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8204476     Medline TA:  Electrophoresis     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  5047-54     Citation Subset:  IM    
Affiliation:
Department of Electrical and Computer Engineering, George Mason University, Fairfax, VA, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Fractionation
Electrophoresis, Microchip / instrumentation*,  methods
Mice
Microelectrodes
Microfluidic Analytical Techniques / instrumentation,  methods*
Microscopy, Fluorescence
NIH 3T3 Cells
Tin Compounds / chemistry*
Chemical
Reg. No./Substance:
0/Tin Compounds; 71243-84-0/indium tin oxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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