Document Detail

Dielectrophoretic Separation of Cancer Cells from Blood.
MedLine Citation:
PMID:  20011619     Owner:  NLM     Status:  Publisher    
Recent measurements have demonstrated that the dielectric properties of cells depend on their type and physiological status. For example, MDA-231 human breast cancer cells were found to have a mean plasma membrane specific capacitance of 26 mF/m(2), more than double the value (11 mF/m(2)) observed for resting T-lymphocytes. When an inhomogeneous ac electric field is applied to a particle, a dielectrophoretic (DEP) force arises that depends on the particle dielectric properties. Therefore, cells having different dielectric characteristics will experience differential DEP forces when subjected to such a field. In this article, we demonstrate the use of differential DEP forces for the separation of several different cancerous cell types from blood in a dielectric affinity column. These separations were accomplished using thin, flat chambers having microelectrode arrays on the bottom wall. DEP forces generated by the application of ac fields to the electrodes were used to influence the rate of elution of cells from the chamber by hydrodynamic forces within a parabolic fluid flow profile. Electrorotation measurements were first made on the various cell types found within cell mixtures to be separated, and theoretical modeling was used to derive the cell dielectric parameters. Optimum separation conditions were then predicted from the frequency and suspension conductivity dependencies of cell DEP responses defined by these parameters. Cell separations were then undertaken for various ratios of cancerous to normal cells at different concentrations. Eluted cells were characterized in terms of separation efficiency, cell viability, and separation speed. For example, 100% efficiency was achieved for purging MDA-231 cells from blood at the tumor to normal cell ratio 1:1 x 10(5) or 1:3 x 10(5), cell viability was not compromised, and separation rates were at least 10(3) cells/s. Theoretical and experimental criteria for the design and operation of such separators are presented.
Peter R C Gascoyne; Xiao-Bo Wang; Ying Huang; Frederick F Becker
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Publication Detail:
Journal Detail:
Title:  IEEE transactions on industry applications     Volume:  33     ISSN:  1939-9367     ISO Abbreviation:  -     Publication Date:  1997  
Date Detail:
Created Date:  2009-12-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101214002     Medline TA:  IEEE Trans Ind Appl     Country:  -    
Other Details:
Languages:  ENG     Pagination:  670-678     Citation Subset:  -    
Section of Experimental Pathology, University of Texas M. D. Anderson Cancer Center, Houston, TX 77030 USA.
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MeSH Terms
Grant Support
R21 CA088364-01//NCI NIH HHS

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