Document Detail


Dielectrophoresis of Caenorhabditis elegans.
MedLine Citation:
PMID:  21225036     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We demonstrate for the first time the dielectrophoretic trapping and manipulation of a whole animal, the nematode Caenorhabditis elegans. We studied the effect of the electric field on the nematode as a function of field intensity and frequency. We identified a range of electric field intensities and frequencies that trap worms without apparent adverse effect on their viability. Worms tethered by dielectrophoresis (DEP) exhibit behavioral responses to blue light, indicating that at least some of the nervous system functions are unimpaired by the electrical field. DEP is useful to dynamically tether nematodes, sort nematodes according to size, and separate dead worms from live ones.
Authors:
Han-Sheng Chuang; David M Raizen; Annesia Lamb; Nooreen Dabbish; Haim H Bau
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-01-11
Journal Detail:
Title:  Lab on a chip     Volume:  11     ISSN:  1473-0189     ISO Abbreviation:  Lab Chip     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-02-01     Completed Date:  2011-06-03     Revised Date:  2012-02-22    
Medline Journal Info:
Nlm Unique ID:  101128948     Medline TA:  Lab Chip     Country:  England    
Other Details:
Languages:  eng     Pagination:  599-604     Citation Subset:  IM    
Affiliation:
Mechanical Engineering and Applied Mechanics, University of Pennsylvania, Philadelphia, PA, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Behavior, Animal / radiation effects
Caenorhabditis elegans / chemistry*,  radiation effects
Electromagnetic Fields
Electrophoresis*
Models, Theoretical
Grant Support
ID/Acronym/Agency:
1R21HG004854-01A1/HG/NHGRI NIH HHS; NS064030/NS/NINDS NIH HHS; R01 NS064030-04/NS/NINDS NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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