Document Detail


Dickkopf-1 inhibits the invasive activity of melanoma cells.
MedLine Citation:
PMID:  22420644     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Background.  Malignant melanoma (MM) has the highest morbidity of any malignant tumour. It is known that the Wnt pathway of enhances the development of melanoma. Dickkopf (DKK)-1, an inhibitor of the canonical Wnt/β-catenin pathway, might also inhibit the development of MM. Aim.  To examine the influence of DKK-1 expression on the invasive activity of MM cells. Methods.  To detect DKK-1 expression level we performed immunohistochemistry on human tissue sections of normal skin, malignant melanoma (MM) in situ, and MM with lymph-node metastasis. To clarify the role that DDK-1 plays in the invasive activity of MM cells, we performed a scratch-wound healing assay and Transwell invasion assay for A375 cells infected with appropriate recombinant adenoviruses, then assessed the migration of these cells. Results.  Fewer DDK1-overexpressing A375 cells than control cells migrated to the scratch-wound area or through the pores of the Transwell membrane to the lower wells. Downregulation of DKK-1 in A375 cells had the opposite effect. Conclusions.  In the present study, we found for the first time that DKK-1 expression inhibits the invasive activity of MM cells.
Authors:
J Chen; H Li; H Chen; D Hu; Q Xing; G Ren; X Luo
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-3-15
Journal Detail:
Title:  Clinical and experimental dermatology     Volume:  -     ISSN:  1365-2230     ISO Abbreviation:  -     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-3-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7606847     Medline TA:  Clin Exp Dermatol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© The Author(s). CED © 2012 British Association of Dermatologists.
Affiliation:
Department of Dermatology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
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