Document Detail


Dicer activity in neural crest cells is essential for craniofacial organogenesis and pharyngeal arch artery morphogenesis.
MedLine Citation:
PMID:  21256960     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
MicroRNAs (miRNAs) play important roles in regulating gene expression during numerous biological/pathological processes. Dicer encodes an RNase III endonuclease that is essential for generating most, if not all, functional miRNAs. In this work, we applied a conditional gene inactivation approach to examine the function of Dicer during neural crest cell (NCC) development. Mice with NCC-specific inactivation of Dicer died perinatally. Cranial and cardiac NCC migration into target tissues was not affected by Dicer disruption, but their subsequent development was disturbed. NCC derivatives and their associated mesoderm-derived cells displayed massive apoptosis, leading to severe abnormalities during craniofacial morphogenesis and organogenesis. In addition, the 4th pharyngeal arch artery (PAA) remodeling was affected, resulting in interrupted aortic arch artery type B (IAA-B) in mutant animals. Taken together, our results show that Dicer activity in NCCs is essential for craniofacial development and pharyngeal arch artery morphogenesis.
Authors:
Xuguang Nie; Qin Wang; Kai Jiao
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-01-21
Journal Detail:
Title:  Mechanisms of development     Volume:  128     ISSN:  1872-6356     ISO Abbreviation:  Mech. Dev.     Publication Date:    2011 Mar-Apr
Date Detail:
Created Date:  2011-03-28     Completed Date:  2011-08-16     Revised Date:  2014-07-04    
Medline Journal Info:
Nlm Unique ID:  9101218     Medline TA:  Mech Dev     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  200-7     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis
Arteries / embryology*,  enzymology
Bone and Bones / embryology,  enzymology
Branchial Region / blood supply,  embryology*,  enzymology
Cartilage / embryology,  enzymology
Cell Movement
Cell Proliferation
Craniofacial Abnormalities / embryology*,  enzymology,  genetics
DEAD-box RNA Helicases / genetics,  metabolism*
Female
Gene Silencing
Head / embryology,  innervation
Male
Mice
Mice, Transgenic
Morphogenesis
Muscle, Skeletal / embryology,  enzymology
Neural Crest / cytology*,  enzymology
Organogenesis
Peripheral Nervous System / embryology,  enzymology
Retrognathia / embryology,  enzymology,  genetics
Ribonuclease III / genetics,  metabolism*
Grant Support
ID/Acronym/Agency:
R01 HL095783/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
EC 3.1.26.3/Dicer1 protein, mouse; EC 3.1.26.3/Ribonuclease III; EC 3.6.4.13/DEAD-box RNA Helicases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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