| Diazoxide Pretreatment Enhances L6 Skeletal Myoblast Survival and Inhibits Apoptosis Induced by Hydrogen Peroxide. | |
| | |
MedLine Citation:
|
PMID: 22262406 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Skeletal myoblast (SKM) transplantation is a promising approach to regenerate tissue and improve the function of the injured heart. However, the number of survival cells transplanted to host myocardium is quite poor due to high rate of apoptosis; diazoxide (DZ) is a highly selective mito-KATP channel opener that may reduce cell apoptosis by relieving reactive oxygen species (ROS) damage. The aim of this study is to explore the protective effects of DZ on L6 SKM damage induced by hydrogen peroxide (H(2) O(2) ) in vitro. Different dose and time of H(2) O(2) and DZ treatment were performed and only 24 hr of 1.00 mmol/L H(2) O(2) treatment and 200 μmol/L DZ pretreatment for 30 min were used for further experiment. L6 SKMs were cultured and divided into control group (no treatment), H(2) O(2) group (24 hr of 1.00 mmol/L H(2) O(2) treatment) and DZ + H(2) O(2) group (pretreated with 200 μmol/L DZ for 30 min before 24 hr of 1.00 mmol/L H(2) O(2) treatment). Compared with control group, H(2) O(2) treatment caused cell damage, increased lactate dehydrogenase release, cell apoptosis, and bax gene expression, while reduced cell proliferation and decreased bcl-2 expression. DZ pretreatment may protect cells from damage induced by H(2) O(2) and reduce cell apoptosis by increasing bcl-2 and decreasing bax expression. DZ pretreatment may also promote cell proliferation measured by both PCNA expression and flow cytometry method. These results suggest that DZ may protect L6 SKMs from damage induced by H(2) O(2) by maintaining integrity of cell membrane, reducing apoptosis and increasing proliferation in vitro. Anat Rec, 2012. © 2012 Wiley Periodicals, Inc. |
| | |
Authors:
|
Su-Xia Shao; Lei Zhang; Hong-Xia Chen; Yan Liu; Jin-Ping Zhang; Wei Chen; Guo-Yu Xue |
Related Documents
:
|
21687406 - The subversion of the immune system by francisella tularensis. 21929466 - Cytotoxic effects and oxidative stress response of six pbde metabolites on human l02 ce... 22147766 - New insights on ox40 in the control of t cell immunity and immune tolerance in vivo. 22180766 - Natural killer cell responses during viral infections: flexibility and conditioning of ... 2354856 - Secretory immune responses in mouse vaginal fluid after pelvic, parenteral or vaginal i... 21687406 - The subversion of the immune system by francisella tularensis. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-1-20 |
Journal Detail:
|
Title: Anatomical record (Hoboken, N.J. : 2007) Volume: - ISSN: 1932-8494 ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
|
Created Date: 2012-1-20 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101292775 Medline TA: Anat Rec (Hoboken) Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2012 Wiley Periodicals, Inc. |
Affiliation:
|
Department of Histology and Embryology, Hebei Medical University, Shijiazhuang City, Hebei Province, People's Republic of China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Two-phase anaerobic digestion of the organic fraction of municipal solid waste (OFMSW): Estimation o...
Next Document: Reprogramming the Genetic Code: From Triplet to Quadruplet Codes.