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DIAZOPHTHALOCYANINS AS REAGENTS FOR FINE STRUCTURAL CYTOCHEMISTRY.
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MedLine Citation:
PMID:  14283629     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This paper reports the synthesis of 14 diazophthalocyanins containing Mg, Cu, or Pb as the chelated metal. To assess the usefulness of these compounds for fine structural cytochemistry, the relative coupling rates with naphthols were tested as well as the solubility of the resulting azo dyes. Three of the diazotates were reacted with tissue proteins in aldehyde-fixed material, and the density increases thus produced were compared in the electron microscope with those produced by staining similarly fixed material with the phthalocyanin dye, Alcian Blue. Finally, one of the diazotates was used as a capture reagent for the demonstration of the sites of acid phosphatase activity with the electron microscope.
Authors:
L W TICE; R J BARRNETT
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of cell biology     Volume:  25     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1965 Apr 
Date Detail:
Created Date:  1965-07-01     Completed Date:  1996-12-01     Revised Date:  2011-09-28    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  23-41     Citation Subset:  OM    
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MeSH Terms
Descriptor/Qualifier:
Acid Phosphatase*
Anti-Infective Agents, Local*
Azo Compounds*
Cell Biology*
Cell Physiological Phenomena*
Chelating Agents*
Copper*
Hydrogen-Ion Concentration*
Indicators and Reagents*
Lead*
Magnesium*
Microscopy, Electron*
Pigments, Biological*
Rats*
Research*
Chemical
Reg. No./Substance:
0/Anti-Infective Agents, Local; 0/Azo Compounds; 0/Chelating Agents; 0/Indicators and Reagents; 0/Pigments, Biological; 7439-92-1/Lead; 7439-95-4/Magnesium; 7440-50-8/Copper; EC 3.1.3.2/Acid Phosphatase
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Journal Information
Journal ID (nlm-ta): J Cell Biol
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
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Copyright © 1965 by The Rockefeller Institute Press
Received Day: 10 Month: 4 Year: 1964
Print publication date: Day: 1 Month: 4 Year: 1965
Volume: 25 Issue: 1
First Page: 23 Last Page: 41
ID: 2106597
PubMed Id: 14283629

DIAZOPHTHALOCYANINS AS REAGENTS FOR FINE STRUCTURAL CYTOCHEMISTRY
Lois Withrow Tice
Russell J. Barrnett
From the Department of Pathology, College of Physicians and Surgeons, Columbia University, New York, and the Department of Anatomy, Yale University School of Medicine, New Haven, Connecticut


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