| DIAZOPHTHALOCYANINS AS REAGENTS FOR FINE STRUCTURAL CYTOCHEMISTRY. | |
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MedLine Citation:
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PMID: 14283629 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This paper reports the synthesis of 14 diazophthalocyanins containing Mg, Cu, or Pb as the chelated metal. To assess the usefulness of these compounds for fine structural cytochemistry, the relative coupling rates with naphthols were tested as well as the solubility of the resulting azo dyes. Three of the diazotates were reacted with tissue proteins in aldehyde-fixed material, and the density increases thus produced were compared in the electron microscope with those produced by staining similarly fixed material with the phthalocyanin dye, Alcian Blue. Finally, one of the diazotates was used as a capture reagent for the demonstration of the sites of acid phosphatase activity with the electron microscope. |
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Authors:
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L W TICE; R J BARRNETT |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of cell biology Volume: 25 ISSN: 0021-9525 ISO Abbreviation: J. Cell Biol. Publication Date: 1965 Apr |
Date Detail:
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Created Date: 1965-07-01 Completed Date: 1996-12-01 Revised Date: 2011-09-28 |
Medline Journal Info:
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Nlm Unique ID: 0375356 Medline TA: J Cell Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 23-41 Citation Subset: OM |
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acid Phosphatase* Anti-Infective Agents, Local* Azo Compounds* Cell Biology* Cell Physiological Phenomena* Chelating Agents* Copper* Hydrogen-Ion Concentration* Indicators and Reagents* Lead* Magnesium* Microscopy, Electron* Pigments, Biological* Rats* Research* |
| Chemical | |
Reg. No./Substance:
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0/Anti-Infective Agents, Local; 0/Azo Compounds; 0/Chelating Agents; 0/Indicators and Reagents; 0/Pigments, Biological; 7439-92-1/Lead; 7439-95-4/Magnesium; 7440-50-8/Copper; EC 3.1.3.2/Acid Phosphatase |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): J Cell Biol ISSN: 0021-9525 ISSN: 1540-8140 Publisher: The Rockefeller University Press |
Article Information Download PDF  Copyright © 1965 by The Rockefeller Institute Press Received Day: 10 Month: 4 Year: 1964 Print publication date: Day: 1 Month: 4 Year: 1965 Volume: 25 Issue: 1 First Page: 23 Last Page: 41 ID: 2106597 PubMed Id: 14283629 |
| DIAZOPHTHALOCYANINS AS REAGENTS FOR FINE STRUCTURAL CYTOCHEMISTRY | |
| Lois Withrow Tice | |
| Russell J. Barrnett | |
| From the Department of Pathology, College of Physicians and Surgeons, Columbia University, New York, and the Department of Anatomy, Yale University School of Medicine, New Haven, Connecticut |
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