Document Detail


Diazeniumdiolate-doped poly(lactic-co-glycolic acid)-based nitric oxide releasing films as antibiofilm coatings.
MedLine Citation:
PMID:  22841918     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Nitric oxide (NO) releasing films with a bilayer configuration are fabricated by doping dibutyhexyldiamine diazeniumdiolate (DBHD/N2O2) in a poly(lactic-co-glycolic acid) (PLGA) layer and further encapsulating this base layer with a silicone rubber top coating. By incorporating pH sensitive dyes within the films, pH changes in the PLGA layer are visualized and correlated with the NO release profiles (flux vs. time). It is demonstrated that PLGA acts as both a promoter and controller of NO release from the coating by providing protons through its intrinsic acid residues (both end groups and monomeric acid impurities) and hydrolysis products (lactic acid and glycolic acid). Control of the pH changes within the PLGA layer can be achieved by adjusting the ratio of DBHD/N2O2 and utilizing PLGAs with different hydrolysis rates. Coatings with a variety of NO release profiles are prepared with lifetimes of up to 15 d at room temperature (23 °C) and 10 d at 37 °C. When incubated in a CDC flow bioreactor for a one week period at RT or 37 °C, all the NO releasing films exhibit considerable antibiofilm properties against gram-positive Staphylococcus aureus and gram-negative Escherichia coli. In particular, compared to the silicone rubber surface alone, an NO releasing film with a base layer of 30 wt% DBHD/N2O2 mixed with poly(lactic acid) exhibits an ∼98.4% reduction in biofilm biomass of S. aureus and ∼99.9% reduction for E. coli at 37 °C. The new diazeniumdiolate-doped PLGA-based NO releasing coatings are expected to be useful antibiofilm coatings for a variety of indwelling biomedical devices (e.g., catheters).
Authors:
Wenyi Cai; Jianfeng Wu; Chuanwu Xi; Mark E Meyerhoff
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-07-28
Journal Detail:
Title:  Biomaterials     Volume:  33     ISSN:  1878-5905     ISO Abbreviation:  Biomaterials     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-08-31     Completed Date:  2013-01-16     Revised Date:  2013-11-06    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  England    
Other Details:
Languages:  eng     Pagination:  7933-44     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Ltd. All rights reserved.
Affiliation:
Department of Chemistry, The University of Michigan, Ann Arbor, MI 48109, USA.
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MeSH Terms
Descriptor/Qualifier:
Anti-Bacterial Agents / administration & dosage*,  pharmacology
Azo Compounds / chemistry*
Biofilms / drug effects*
Coated Materials, Biocompatible / chemistry
Escherichia coli / drug effects,  physiology*
Escherichia coli Infections / prevention & control
Humans
Hydrogen-Ion Concentration
Lactic Acid / chemistry*
Nitric Oxide / administration & dosage*,  pharmacology
Polyglycolic Acid / chemistry*
Prosthesis-Related Infections / prevention & control
Silicone Elastomers / chemistry
Staphylococcal Infections / prevention & control
Staphylococcus aureus / drug effects,  physiology*
Grant Support
ID/Acronym/Agency:
EB-000783/EB/NIBIB NIH HHS; EB-004527/EB/NIBIB NIH HHS; R01 EB000783/EB/NIBIB NIH HHS; R01 EB004527/EB/NIBIB NIH HHS
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Azo Compounds; 0/Coated Materials, Biocompatible; 0/Silicone Elastomers; 0/diazeniumdiolate; 0/polylactic acid-polyglycolic acid copolymer; 10102-43-9/Nitric Oxide; 26009-03-0/Polyglycolic Acid; 50-21-5/Lactic Acid
Comments/Corrections

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